固相萃取-高效液相色谱法测定肉制品中苯并(a)芘

Determination of Benzo(a)pyrene in Meat Products by HPLC with Solid-Phase Extraction

建立固相萃取-高效液相色谱法测定肉制品中苯并(a)芘的检测方法。样品经Cleanert BaP固相萃取柱净化、浓缩,Waters SunFire C18(4.6mm×250mm,5μm)色谱柱进行分离,流动相为乙腈-水(88:12,V/V),流速1.0mL/min,荧光检测器,激发波长386nm,发射波长405nm。结果表明,苯并(a)芘在线性范围0.5～10ng/mL内,相关系数为0.9978,最低检出限为0.04μg/kg,精密度(RSD)小于5%,回收率82.2%～94.9%。本方法快速简便、重现性好,可以用于肉制品中苯并(a)芘含量的测定,为肉制品的质量控制和安全评价提供保证。

A high performance liquid chromatography with solid-phase extraction method was developed for the determination of benzo（a）pyrene in meat products. The extracted sample was purified on a Cleanert BaP solid-phase extraction column and separated on a Waters SunFire CIs column （4.6mm×250mm,5μm） using a mobile phase composed of acetonitrile and water （88/12） at a flow rate of 1.0 mL/min. Benzo（a）pyrene was measured using a fluorescence detector at an excitation wavelength of 386 nm and an emission wavelength of 405 nm. The result showed that benzo（a）pyrene displayed good linearity relationship over the concentration range of 0.5-- 10 ng/mL, with a coefficient correlation of 0.9978. The limit of detection was 0.04 μg/kg, and the precision （RSD） for 6 replication determinations was below 5%. The average blank spike recoveries across the levels was in the range of 82.2% -- 94.9% （n = 3）. This method was rapid, simple, reproducible, and applicable for benzo（a）pyrene determination in meat products, providing guarantee for quality control and safety evaluation of meat products.