瓶装饮料中DNOP、DBP类增塑剂的液相色谱检测方法

The HPLC Detection Method of DNOP and DBP in the Beverage of Plastic Bottle Packing

邻苯二甲酸酯类是一类重要的增塑剂,也是环境类激\素,会影响动物内分泌系统;对人不仅有慢性毒性,致突变、致癌作用,还有生殖、发育毒性。本文研究高效液相色谱检测瓶装饮料中两种邻苯二甲酸酯(DNOP、DBP)的方法。样品经过滤、浓缩后过柱净化、富集,用50℃氮吹仪吹干,甲醇定容后过膜(0.22μm),在UV 225nm处检测。以ProELUT PLS GlASS 200mg/6mL小柱作为样品前处理净化柱,结果对DBP的加标回收率在83.4%～93.4%之间,对DBP的加标回收率在80.4%～86.0%之间。本实验条件下DBP和DNOP的仪器检出限均为0.01μg/mL,说明用液相色谱法检测瓶装饮料中邻苯二甲酸酯类物质含量是可行的,且快速、有效。

Phthalate esters（PAEs） were a class of important plasticizers, and it also were the environmental estrogens, it affects the animal endocrine system. PAEs were not only with chronic toxic, mutagenic, carcinogenic effects to people, but also with reproductive and upgrowth toxicity. This study focused on the method of detection of DNOP and DBP in plastic packing beverage by HPLC. The sample were filtrated and enrichment by clean up column, and nitrogen drying at 50 ℃, and pass through 0.22 μm film after fixed volume with methanol, and detected at 225 nm by HPLC. ProELUT PLS GlASS 200 mg/6 mL as the clean up column, the recovery rate of DBP was 83.4%～93.4%, DNOP was 80.4%～86.0%. The detection limit of instrument was 0.01 μg/mL. The results showed that the HPLC method was effective and convenient for the detection of DNOP and DBP in beverage.