低剂量接触镉对未成年大鼠卵巢发育影响

Impact of low dose cadmium on development of immature female ovary

目的观察镉对卵泡生长发育和性激素分泌影响,探讨镉对未成年雌性大鼠卵巢发育的毒性作用。方法未成年雌性清洁级Wistar大鼠48只,按体重随机分为4组,对照组(生理盐水)和低、中、高剂量镉染毒组(0.1、0.3、0.9 mg/kg),腹腔注射染毒,1次/d,连续19 d;染毒结束后,捡测大鼠血清雌激素(E2)、孕激素(P4)水平;取出大鼠卵巢组织连续切片观察卵巢各级卵泡数目构成比。结果低、中、高剂量染镉组血清中E2的水平分别为(6.04±0.28)、(6.10±0.13)和(5.96±0.18)pg/mL,均低于对照组的(6.62±0.24)pg/mL,差异均有统计学意义(P<0.05);高剂量染镉组大鼠各期卵泡数目构成比与对照组比较,差异均有统计学意义(P<0.05)。结论在本实验条件下低剂量镉暴露对未成年雌性大鼠的卵巢发育具有毒性作用。

Objective To study the effects of cadmium exposure on expression of hormone,including estradiol （ E2 ） and progesterone（ P4 ） and follicle development and to elucidate whether cadmium has toxicity in immature ovary. Methods Totally 48 Wistar rats were exposed to cadmium by intraperitoneal injected at the dose of 0,0. 1,0. 3 and 0. 9 mg Cd2＋/kg body weight,once a day for 19 days. After the exposure ,the rats＇ ovaries were prepared to detect expression of follicles. The sera of the rats were sampled to determine E2 and P4 levels. Results E2 levels in each dosage groups （6. 04 ±0. 28,6. 10 ±0. 1 ,and 5.96 ±0. 18 pg/mL） were statistically significantly lower than that of the control group （ 6. 62 ± 0. 24 pg/mL ,P 〈 0. 05 ）. The follicle expression in 0. 9 mg Cd2＋/kg group was different from that of the control group （ P 〈 0. 05 ）. Conclusion Cadmium has obvious toxicity to immature female ovary development.