TWIST、HIF-1α、VEGF在胃癌中的表达及意义

Expression of TWIST,HIF-1α and VEGF in gastric cancer and their significance

目的:探讨TWIST,HIF-1α和VEGF在人胃癌组织中的表达及与临床病理参数、预后的关系。方法:选择120例胃癌标本,应用SP法免疫组化检测TWIST、HIF-1α和VEGF在人胃癌组织中的表达,分析TWIST、HIF-1α和VEGF的表达与患者临床病理参数之间的关系。结果:胃癌组织及正常胃黏膜组织中均可检测到TWIST、HIF-1α和VEGF的表达,但胃癌组织中的表达水平均明显高于正常胃黏膜组织,表达差异有显著性(P<0.05)。TWIST的表达与浸润深度、肿瘤远处转移及淋巴结转移明显相关(P<0.05)。HIF-1α的表达与肿瘤的分化程度、浸润深度及淋巴结转移呈明显相关性(P<0.05)。VEGF的阳性表达与浸润深度、淋巴结转移及TNM分期有关(P<0.05)。TWIST和(或)HIF-1α阳性表达的患者其5年生存率明显低于其阴性表达的患者。结论:胃癌中TWIST、HIF-1α和VEGF的高表达与胃癌的生物学行为及预后密切相关,检测其表达对预测胃癌的转移及判断预后有一定价值。

Objective：To investigate the relationship between the expressions of TWIST,HIF-1α,VEGF and their clinical pathological parameters in gastric carcinoma.Explore their significances in judging the prognosis of patients.Methods：Immuohistochemistry was used to detect the expressions of TWIST,HIF-1α and VEGF in 120 cases of gastric carcinoma specimens and corresponding adjacent normal tissue.Results：TWIST,HIF-1α and VEGF expression could be detected in gastric cancer tissues and normal gastric mucosa,but expression in gastric cancer tissues was significantly higher than in normal gastric mucosa,expression differences were significant（P0.05）.Patients with TWIST positive expression showed higher incidence of lymph node metastasis（P0.05） and distant metastasis（P0.05） than those with TWIST negative expression.HIF-1α expression was closely correlated with tumor histological type,depth of invasion,lymph node metastasis（P0.05）.The expression of VEGF was significant related with depth of invasion,lymph node metastasis and TNM stage（P 0.05）.Patients with high expressions of TWIST and（or） HIF-1 α had lower five-year survival rate than patients with negative expression.Conclusion：The expressions of TWIST,HIF-1 α and VEGF were closely related to the biological characteristics in gastric carcinoma.It is valuable to detect their expressions for the prediction of gastric carcinoma and judgment of prognosis.