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缺氧对滋养细胞Notch信号通路的影响 被引量：3

Effect of hypoxia on Notch signaling of trophoblasts
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摘要 目的探讨缺氧条件下Notch信号通路在滋养细胞中的表达改变。方法利用实时荧光定量PCR(RT-qPCR)技术检测二氯化钴(CoCl2)化学缺氧条件下人早孕绒毛滋养细胞株(the human first-trimester extravillous tropho-blast cell line,TEV-1)中Notch1及Jagged1 mRNA的表达情况。结果 (1)与正常对照组相比,缺氧条件下滋养细胞Notch1 mRNA表达量无明显变化(P>0.05)。(2)随着缺氧时间的延长,滋养细胞Jagged1 mRNA表达量逐渐降低,与正常对照组相比具有统计学意义(P<0.05)。结论缺氧条件下滋养细胞Notch1及Jagged1表达平衡失调,可能参与滋养细胞功能调控。 Objective ： To investigate the change of Notch signaling in hypoxic trophoblasts. Methods ： By using quantitative real - time PCR, we detected the expression of Notchl and Jaggedl in human extravillous trophoblast cell line TEV - 1, which was cultured under Cobalt chloride （ CoCl2 ） in vitro. Results ： （ 1 ） The mRNA expression of Notchl in control groups showed no difference as compared with hypoxic groups （P 〉 0. 05 ）. （2） The mRNA expression of Jaggedl was gradually reduced with the extending of time and was significantly decreased as compared with hypoxic groups （ P 〈 0. 05 ）. Conclusions ： The imbalance expression of Notchl and Jaggedl in hypoxic trophoblasts may contribute to functional regulation of trophoblasts.
作者 张娜 吴媛媛 刘海意 龚洵 石鑫玮 李宇琪 乔福元
机构地区 华中科技大学同济医学院附属同济医院妇产科
出处 《中国优生与遗传杂志》 2012年第8期15-16,14,共3页 Chinese Journal of Birth Health & Heredity
基金 高等学校博士学科点专项科研基金(20100142110074)
关键词 NOTCH1 JAGGED1 缺氧 滋养细胞 Notchl Jaggedl Hypoxia Trophoblasts
分类号 R363 [医药卫生—病理学]
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参考文献8

  • 1Cobellis L, Mastrogiacomo A, Federico E et al. Distribution of Notch protein members in normal and preeclampsia- complicated placentas [J]. 2007,330:527 -534.
  • 2Zeliha Sahin, Nuray Acar, Ozlem Ozbey, et al. Distribution of Notch family proteins in intrauterine growth restriction and hypertension complicated human term placentas [ J ]. 2011,113 : 270 - 276.
  • 3Feng HC, Choy MY, Deng W, et al. Establishment and Characteriza- tion of a Human First - Trimester Extravillous Trophoblast Celt Line (TEV - 1 ) [Jl. J Soc Gynecol Investig,2005,12: e21 -32.
  • 4Gerry Weinmaster. Notch signaling: direct or what? [J]. Curr Opin Genet Dev,1998,8: 436 -442.
  • 5Mumm JS, Kopan R. Notch signaling : from the outside in [ J ]. Dev Biol,2000,228: 151 - 165.
  • 6Kopan R, Ilagan MX. The canonical Notch signaling pathway: unfol- ding the activation mechanism[J]. Ce11,2009,137:216-233.
  • 7MDe Falco,LCobellis,DGiraldi, etal. Expression and distribution of notch protein members in human placenta throughout pregnancy [ J ]. Placenta,2007,28 : 118 - 126.
  • 8FHerr, I, Schreiner, NBaal, et al. Expression patterns of Notch recep- tors and their ligands Jagged and Delta in human placenta [ J ]. Pla- centa,2011,32: 554 - 563.

同被引文献22

  • 1Regal JF, Gilbert JS, Burwick RM. The complement system and ad- verse pregnancy outcomes[ J]. Mol Immunol, 2015,67( 1 ) :56-70.
  • 2O'Tiemey-Ginn PF, Lash GE. Beyond pregnancy: modulation of trophoblast invasion and its consequences for fetal growth and long- term children' s health [ J ]. J Reprod Immunol, 2014, 104-105 : 37-42.
  • 3Lee BY, Timpson P, Horvath LG, et al. FAK signaling in human cancer as a target for therapeutics[ J]. Phannacol Ther, 2015,146: 132-149.
  • 4Yoon H, Dehart JP, Murphy JM, et al. Understanding the roles of FAK in cancer : inhibitors, genetic models, and new insights [ J ]. J Histochem Cytochem ,2015,63 ( 2 ) : 114-128.
  • 5Zhang J, Ren R, Luo X, et al. A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/ SVneo cells : involvement of activation of focal adhesion kinase signa-ling[J]. PLoS One, 2014, 3(9):e92252.
  • 6Jiang R, Cai J, Zhu Z, et al. Hypoxic trophoblast HMGB1 induces endothelial cell hyperpermeability via the TRL-4/caveolin-1 pathway [J]. J Immunol, 2014,193 (10) : 5000-5012.
  • 7Tang CL, Zhao HB, Li MQ, et al. Focal adhesion kinase signaling is necessary for the Cyclosporin A-enhanced migration and invasion of human trophoblast cells[J]. Placenta, 2012.33 (9) :704-711.
  • 8Tamaru S, Mizuno Y, Tochigi H, et al. MicroRNA-135b suppres- ses extravillous trophoblast-derived HTR-8/SVneo cell invasion by directly down regulating CXCL12 under low oxygen conditions[ J]. Biochem Biophys Res Commun, 2015, 461 (2) :421-426.
  • 9Schanz A, Red-Horse K, Hess AP, et al. Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor ex- oression[ J]. Placenta,2014,35 (12) :1089-1094.
  • 10Zhang Z, Zhang L, Jia L,et al. AP-2α suppresses invasion in Be- Wo cells by repression of matrix metalloproteinase-2 and -9 and up- regulation of E-cadherin [ J ]. Mol Cell Biochem, 2013, 381 ( 1-2 ) : 31-39.

引证文献3

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中国优生与遗传杂志
2012年 第8期

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