紫花牡荆素抑制乳腺癌MCF-7细胞增殖与侵袭能力的研究

Casticin inhibits proliferation and invasion of breast cancer MCF-7 cells

目的观察紫花牡荆素对乳腺癌MCF-7细胞株增殖与侵袭能力的影响并探讨其分子机制。方法应用四甲基偶氮唑蓝（MTT）比色法与侵袭实验检测紫花牡荆素对MCF-7细胞增殖与侵袭能力的影响，应用反转录PCR、Western blot法、Tunel法检测紫花牡荆素对基因表达、蛋白表达、细胞凋亡的影响。结果不同浓度紫花牡荆素均抑制MCF-7细胞增殖水平，同未加药对照组比较差异均有统计学意义（P〈0．05），5、10、20μmol／L紫花牡荆素作用后，MCF-7细胞迁移数与未处理组相比分别降低20.3％、44．4％和50．3％（P〈0．05）。以10μmol／L紫花牡荆素处理后，凋亡细胞数增多，可以上调Bax与Caspase-3蛋白的表达水平，下调基质金属蛋白酶（MMP）-2与MMP-9的mRNA和蛋白表达水平。结论紫花牡荆素对于乳腺癌细胞恶性增殖与侵袭能力具有显著抑制作用。

Objective To investigate the effects of casticin on proliferation and invasion on human breast cancer MCF-7 cells. Methods MTT assay and invasion assay were performed in MCF-7 cells treated with casticin, respectively. The expression of Bax, Caspase-3, MMP-2 and MMP-9 was analyzed by RT-PCR and Western blot. Results Casticin inhibited MCF-7 cells proliferation and invasion in a dose-dependent manner （P 〈 0.05）. Additionally, Bax and Caspase-3 expression was increased in MCF-7 cells treated with casticin, whereas MMP-2 and MMP-9 were down-regulated. Conclusion Casticin may play a critical role in inhibiting breast tumor cell proliferation and invasion, reinforcing its potential in clinical application.