电针足三里穴对不全性肠梗阻模型大鼠小肠Cajal间质细胞数目和网络结构的影响

Influence of electroacupuncture in Zusanli(ST36) on number and network of interstitial cells Cajal of small intestine in rats with partial intestinal obstruction

目的观察电针足三里穴对不全性肠梗阻模型大鼠小肠Cajal间质细胞(ICCs)数目和网络结构的影响,为进一步研究电针足三里穴对ICCs表型变化的影响奠定基础。方法采用末端回肠套环法建立大鼠不全性肠梗阻模型,将大鼠随机分为:正常对照组、假手术组、未电针组、电针足三里穴(ST36)组(简称足三里组)、电针阴陵泉穴(GB34)组(简称阴陵泉组)。造模成功后,足三里组和阴陵泉组给予电针干预治疗7 d后,冰冻切片和全层铺片免疫细胞化学染色观察各组大鼠结肠ICCs的数目及分布变化情况。结果未电针组与正常对照组和假手术组比较,小肠梗阻前端和梗阻处ICCs呈现不连续分布,网络结构被破坏,数目显著减少(P<0.05)。足三里组较未电针组,ICCs分布呈连续性,网络结构有所修复且数目显著增加(P<0.05);而阴陵泉组与未电针组比较,变化不显著(P>0.05)。结论采用末端回肠套环法能有效建立大鼠不全性肠梗阻模型,电针足三里穴能在一定程度上刺激ICCs再生和恢复成正常的细胞网络结构,从而促进回肠功能恢复。

Objective To observe the influence of electroacupuncture in Zusanli（ST36） on number and network of interstitial cells Cajal（ICCs） of small intestine in rats with partial intestinal obstruction,and lay a foundation for further study on the influence of electroacupuncture in ST36 on phenotypic variation of ICCs.Methods The rat model of partial intestinal obstruction was established by using terminal ileum ringer method.All rats were randomly divided into normal group,sham-operation group,non-electroacupuncture group（non-EA group）,electroacupuncture in Zusanli（ST36） group（ST36 group） and electroacupuncture in Yinlingquan（GB34） group（GB34 group）.After success modeling,ST36 group and GB34 group were given electroacupuncture intervention for 7 days.The changes of number and distribution of ICCs in colon were observed in all groups by applying the technique of frozen sections and whole-mount preparation of immunocytochemical staining.Results The comparison between non-EA group and normal group or sham-operation group showed a discontinuous distribution of ICCs,damaged cellular networks and reduced number（P0.05）.The comparison between ST36 group and non-EA group showed a continuous distribution of ICCs,recovered cellular network and significantly increased number（P0.05）.The comparison between GB34 group and non-EA group showed no significant changes（P0.05）.Conclusion The method of terminal ileum ringer can by used for establishing effectively rat model of partial intestinal obstruction.Electroacupuncture in ST36 can stimulate to some extent the regeneration of ICCs to recover to normal cellular network structure,and improve the recovery of ileum function.