检测活性氧物种的氧杂蒽类光学探针的研究进展

Progress in Xanthene-based Spectroscopic Probes for Reactive Oxygen Species

活性氧物种在维持生物体的生理功能方面发挥着重要的作用。高于正常水平的活性氧物种会损伤蛋白质、DNA等生物分子,进而导致疾病。因此,活性氧物种的高选择性、高灵敏度检测研究对疾病的预防、诊断和治疗均具有重要意义。荧光探针因具有分析灵敏度高、样品时空分辨能力强等特点,已在该方面获得了广泛的应用。其中,具有发射波长长,光稳定性好,荧光量子产率高等优点的氧杂蒽类荧光探针已成为检测活性氧物种的研究热点。本论文主要总结了近五年来应用于活性氧物种检测的氧杂蒽类荧光探针的研究进展与成像分析,归纳了不同活性氧物种的识别单元,并展望了此类探针的发展趋势与应用前景。

Reactive oxygen species （ROS） play key roles in maintaining normal physiological function of organisms. Elevated level of ROS in vivo could damage various biological molecules, such as proteins and DNA, leading to diseases. Therefore, highly selective and sensitive measurement of ROS is of great importance for prevention, diagnosis, and treatment of diseases. In this respect, fluorescent probes have been widely used due to their high spatial and temporal resolution capability. In recent years, xanthene-based spectroscopic probes for ROS have become a research focus, because of their excellent properties, such as relatively long emission wavelengths, good photostability and high quantum yields. Herein, we mainly review the progress of the xanthene-based spectroscopic probes for ROS over the past five years, including the detection and fluorescence imaging of hydrogen peroxide, hypochlorous acid/hypochlorite, superoxide, hydroxyl, singlet oxygen, nitric oxide, and so on. The recognition reactions used in constructing spectroscopic probes for different ROS are first summarized, and the developing trends and prospects of xanthene-based spectroscopic probes in the future are discussed.