伊班膦酸钠对大鼠正畸源性牙移动及牙根吸收影响研究

Effect of ibandronate on orthodontic root resorption in rats

目的探讨伊班膦酸钠对正畸源性牙根吸收的影响。方法本实验于2011年6月至2012年1月在山东大学西校区动物实验中心完成。选用30只6周龄雌性SPF级Wistar大鼠,随机分为实验组和对照组,各15只。建立大鼠正畸牙移动模型,安装矫治器前3d实验组于腭侧黏骨膜下局部注射伊班膦酸钠50μL,对照组于相同部位注射生理盐水50μL,每3d注射1次直至实验结束。2组动物分别于安装矫治器后第3、7、14d各处死5只。检测大鼠第一磨牙近中移动距离、牙根吸收指数,并进行牙体牙周联合切片的组织学观察。结果安装矫治器后第7、14d时实验组牙齿移动距离、牙根吸收指数、破牙骨质细胞数及破骨细胞分化因子阳性表达均小于对照组,差异具有统计学意义(P<0.05)。结论局部应用伊班膦酸钠可抑制大鼠正畸牙齿的移动及牙骨质和牙本质的异常吸收,利于牙周组织受正畸力后的改建。

Objective To investigate the effect of ibandronate on orthodontic root resorption in rats. Methods Thirty 6-week-old SPF female Wister rats were randomly divided into two groups of fifteen each： the control group and the ibandronate group. Establish the experimental model of the same appliance with 40 gram forces by operation. The ibandro- nate group was injected with 50ul of ibandronate on the first permanent molars in the palatal mucoperiosteal; the control group was injected with 0.9% normal saline 50μL, injected every 3 days since beginning injecting before establishing rat model for 3 days. Thirty rats were killed separately on 3,7 and 14 days. The tooth movement distances and the root resorption index were measured and periodontal tissues were studied histologically. Results The distance and the root resorption index of the ibandronate group was significantly smaller than the control group （P 〈 0.05 ） on 7 and 14 days. The cementoclasts on molar pressure side of the ibandronate group was significantly less than the control group （P 〈 0.05 ） on 7 and 14 days. The mean optical density value with ODF dyeing of the ibandronate group was significantly less than the control group （P 〈 0.05） on 7 and 14 days. The peridental membrane condition of ibandronate group had significant improvement. Conclusion Ibandronate may inhibit abnormal resorption of cementum and dentin; ibandronate may slow the tooth movement of rats and improve the remodelling of powered periodontal tissue.