舒肝袪脂胶囊中羟基红花黄色素A和大黄素的HPLC法测定

Determination of Hydroxysafflor Yellow A and Emodin in Shuganquzhi Capsules by HPLC

建立了高效液相色谱法同时测定舒肝袪脂胶囊中的羟基红花黄色素A和大黄素。采用C18色谱柱,以甲醇-1%乙酸为流动相,梯度洗脱,检测波长254 nm。羟基红花黄色素A和大黄素在0.1～60 g/ml和0.1～6 g/ml浓度范围内线性关系良好,平均回收率大于98%,日内和日间RSD均小于2.0%。

An HPLC method was established for the simultaneous determination of hydroxysafflor yellow A and emodin in Shuganquzhi capsules. A C18 column was used with the mobile phase of methanol -1% acetic acid by gradient elution at the detection wavelength of 254 nm. The calibration curves of hydroxysafflor yellow A and emodin were linear in the concentration ranges of 0.1 - 60 μg/ml and 0.1 - 6 μg/ml, respectively. The recoveries were over 98 %, with intra- and inter-day RSDs less than 2.0%.