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HIWI在食管癌细胞系Eca-109中的表达及意义 被引量：2

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摘要 目的检测干细胞基因HIWI在食管癌细胞系Eca-109中的表达及其对细胞增殖的影响。方法通过细胞免疫荧光检测食管癌细胞系Eca-109中HIWI蛋白的表达;应用LipofectamineTM2000将HIWI特异的siRNA转染进细胞中,再通过RT-PCR检测siRNA的沉默效率;通过MTT检测转染后各转染组细胞的生长曲线,观察HIWI对食管癌细胞增殖的影响。结果 HIWI蛋白在食管癌细胞系Eca-109中高表达,且细胞之间存在差异;HIWI特异的siRNA沉默效率可高达80%左右;转染测得HIWI特异siRNA组较各对照组细胞的增殖速度减慢并有统计学意义。结论 HIWI在食管癌细胞系Eca-109中高表达,细胞中存在食管癌干细胞;降低HIWI的表达后能够减慢食管癌细胞的增殖速度。
作者 刘志良 石磊芝 杜亚明
机构地区 辽宁医学院附属第一医院 浙江医院
出处 《山东医药》 CAS 2012年第32期43-45,I0003,共4页 Shandong Medical Journal
基金 辽宁省科技厅科技攻关项目计划项目(2011225015) 辽宁医学院青年科技启动基金项目(Y2011Z001) 辽宁医学院附属第一医院青年科技启动基金项目(Fy2011-01)
关键词 食管肿瘤 HIWI 肿瘤干细胞 SIRNA
分类号 R735.1 [医药卫生—肿瘤]
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参考文献8

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同被引文献48

  • 1汪建林,孙志强,于静萍,等食管癌细胞株中肿瘤干细胞样细胞培养分离及生物学鉴定[C].第八届中国肿瘤学术大会暨第十三届海峡两岸肿瘤学术会议论文集.2014:940.
  • 2Kammori M, Izumiyama N, Nakamura K, et al. Telomere metabolism and diagnostic demonstration of telomere measurement in the human esophagus for distinguishing benign from malignant tissue by tissue quantitative fluorescence in situ hybridization. Oncelogy. 2006;71(5-6):430-436.
  • 3Chen B, Fang WK, Wu ZY, et al. The prognostic implications of microvascular density and lymphatic vessel density in esophageal squamous cell carcinoma: Comparative analysis between the traditional whole sections and the tissue microarray. Acta Histochem. 2014;116(4):646-653.
  • 4Yang Y, Wang L, Wang S, et al. Study of metabonomic profiles of human esophageal carcinoma by use of high-r.esolution magic-angle spinning IH NMR spectroscopy and multivariate data analysis.Anal Bioanal Chem. 2013;405(10):3381-3389.
  • 5Sun LL, Sun XX, Xu XE, et al. Overexpression of Jumonji AT-rich interactive domain 1B and PHD finger protein 2 is involved in the progression of esophageal squamous cell carcinoma. Acta Histochem. 2013; 115(1 ):56-62.
  • 6Chen J, Pan J, Zheng X, et al. Number and location of positive nodes, postoperative radiotherapy, and survival after esophagectomy with three-field lymph node dissection for thoracic esophageal squamous cell carcinoma. Int J Radiat Oncol Biol Phys. 2012;82(1):475-482.
  • 7Zhang J, Yang Y, Chen L, et al. Overexpression of pituitary tumor transforming gene (PTTG) is associated with tumor progression and poor prognosis in patients with esophageal squamous cell carcinoma: Acta Histochem. 2014; 116(3): 435-439.
  • 8李林,田辉,岳韦名,等.人间充质干细胞对肿瘤细胞A549和Eca-109增殖、凋亡和侵袭影响的实验研究[C].山东省第十一次胸心血管外科学学术会议论文集.2011:458-472.
  • 9Kim MK, Cho K J, Park SI, et al. Initial stage affects survival even after complete pathologic remission is achieved in locally advanced esophageal cancer: analysis of 70 patients with pathologic major response after preoperative chemoradiotherapy. Int J Radiat Oncol Biol Phys. 2009;75(1): 115-121.
  • 10Zhong X, Yu J, Zhang B, et al. Usfng 18F-fluorodeoxyglucose positron emission tomography to estimate the length of gross tumor in patients with squamous cell carcinoma of the esophagus. Int J Radiat Oncol Biol Phys. 2009;73(1): 136-141.

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山东医药
2012年 第32期

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