传染性胰脏坏死病毒逆转录聚合酶链式反应法(RT-PCR)的研究

Studies on Reverse Transcription PCR Method for Infectious Pancreatic Necrosis Virus

本文针对IPNVA片段VP5基因和VP3基因分别设计了两对特异性引物,同时优化了反应体系和反应条件,建立了检测IPNV逆转录聚合酶链式反应法。并用优化好的反应条件对从人工感染的斑马病鱼中提取的核酸进行扩增,分别扩增出了224bp和206bp的目的条带。

Two pairs of specific primers were designed based on VP5 and VP3 gene of IPNV fragment A, and the reaction system and conditions were optimized to establish a RT-PCR assay for detection of IPNV. And the optimized reaction system was used to amplify the nucleic acid extracted from the artificially infected Zebra diseased fish, gaining the target bands of 224 bp and 206 bp. The detection sensitivity was lpg.