橘全爪螨不同品系γ氨基丁酸受体基因差异性分析

Difference analysis of GABA receptor gene between different strains in the red mite,Panonychus citri(Acari: Tetranychidae) of citrus

【目的】为明确γ氨基丁酸受体基因与橘全爪螨抗性的关系,【方法】通过BLAST搜索,对GABA受体基因进行鉴定;进一步通过序列比对和Sanger测序,对橘全爪螨敏感品系和噻螨酮抗性品系GABA受体基因SNPs进行分析和真实性验证;采用RPKM法对橘全爪螨敏感品系和噻螨酮抗性品系GABA受体基因进行表达差异分析。【结果】从橘全爪螨转录组中获得了19条GABA受体基因。通过敏感品系和抗性品系GABA受体基因序列比较和sanger测序发现Unigene11199_All有2个SNP位点。对GABA受体基因表达差异分析发现,相对于敏感品系,抗性品系中部分GABA受体基因表达量发生了不同程度的变化,抗性品系中有3条GABA受体基因表达上调,13条GABA受体基因表达下调,Unigene24440_All下调倍数最高[log2 Ratio(RS/SS)=-10.452479]。【结论】由此推断,橘全爪螨抗性产生可能是一个比较复杂的过程,而Unigene11199_All和Unigene24440_All可能是橘全爪螨对噻螨酮产生抗性的重要原因。

[Objective]In order to clarify the relationship between T-aminobutyric acid receptor gene and resistance of Panonychus citri. [Method]GABA receptor genes were identified by BLAST searching. SNPs in GABA receptor genes between susceptible strain and hexythiazox-resistant strain of P. citri were ana- lyzed and examined by comparison of GABA receptor gene sequences and Sanger sequencing, and gene expression profiles of GABA receptor genes were further compared using RPKM （Reads Per kb per Million reads） method. [Result] 19 GABA receptor genes were found in transcripts of P. citri. Comparisons of GA- BA receptor gene sequences and further sequencing showed that there were 2 SNPs in Unigenel 1199_All between different strains. Analysis of difference of gene expression showed 3 GABA receptor genes were up-regulated and 13 were down-regulated, of which, Unigenel1069_All was the top down- regulated GABA receptor gene [log2 Ratio（RS/SS）= -10.452479]. [Conclusion]The above results indicated that acaricide resistance may be a complex biological process, and Unigenell199_All and Unigenel1069_All played an important role in hexvthiazox resistance of P. citri.