针刺穴位与非穴位筋膜对酒精性肝纤维化模型大鼠干预的比较研究

Experimental study on the effect and intervention of connective tissues in alcohol-induced liver fibrosis in rats

目的:通过针刺穴位筋膜与非穴位筋膜干预的比较,探讨筋膜在防治肝脏纤维化中可能的作用机制,为筋膜学基础研究提供实验依据。方法:针刺酒精性肝纤维化模型大鼠穴位筋膜(穴位组)与非穴位筋膜(筋膜组),测定血清透明质酸(HA)、层连蛋白(LN)、Ⅲ型前胶原(PC-Ⅲ);天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、甘油三酯(TG)的含量,并对肝组织进行HE、Mallory染色比较。结果:模型组血清学指标与筋膜组、穴位组相比均有显著性差异(P<0.05),筋膜组与穴位组相比,其血清中LN、PC-Ⅲ、AST、TG高于穴位组,且有显著性差异(P<0.05);模型组肝HE、Mallory染色与筋膜组、穴位组相比形态结构均有差异,筋膜组与穴位组相比形态结构无明显差异。结论:针刺穴位筋膜与非穴位筋膜均具有防治肝纤维化模型大鼠功效,且针刺穴位筋膜功效优于针刺非穴位筋膜。

Objective： To study the effect of connective tissues to hepatic fibrosis, by acupuncturing point connective tissues and non-point connective tissues, which may became a basic research for the connective tissues to provide experimental evidence. Methods： Rat model of liver fibrosis was induced by intragastric infusion of a mixture of alcoholic liquid, and acupuncturing point connective tissues and non-point connective tissues. We detected the concentration of HA, LN, AST, ALT, TG and PC-Ill in serum respectively 17 weeks later. Animals were sacrificed, and livers were harvested for morphology inspection. Results： The serum level of the model group was significantly different from the other groups（P〈0.05）, and the formation of liver fibrosis was successfully ameliorated. The serum level of LN, PC-Ifl, AST, TG of the connective tissues group were higher than the tradition point group（P〈0.05）. And the same results were in morphology inspection. Conclusion： Connective tissues play a part in preventing and curing hepatic fibrosis by acupuncture, with the results of the connective tissue group were worse than which of the tradition point group.