养心通脉有效部位方动员骨髓间充质干细胞归巢大鼠梗死心肌的实验研究

Experimental study of Yangxin Tongmai Formula effective components recipe promoting bone marrow mesenchymal stem cells homing to infarcted myocardium in rats

目的:观察养心通脉有效部位方(apr-YTF)对大鼠骨髓间充质干细胞(BMSCs)归巢梗死心肌的影响。方法:将急性心肌梗死大鼠模型随机分成apr-YTF注射液、rhG-CSF注射液和PBS缓冲液3组,测量各组大鼠干预后在体心功能、心室肌瘢痕面积、瘢痕毛细血管数、心肌CD34+细胞、Brdu与cTn I双染阳性细胞数、Ⅷ因子、VEGF、bFGF、Flk-1、外周血CD34+细胞数,分析组间差异。结果:①与PBS组比较,apr-YTF组和rhG-CSF组的LVSP、LVEDP、+dp/dtmax、-dp/dtmax均有显著改善,心室肌瘢痕面积显著减小,心室肌毛细血管数、心肌边缘区和外周血液CD34+染色阳性细胞数、心肌梗死边缘区中Brdu与cTn I双染阳性细胞数及Ⅷ因子、Flk-1、VEGF、bFGF的表达均显著增加(P<0.01,P<0.05);②与rhG-CSF组比较,apr-YTF组的LVSP显著升高,心肌组织CD34+染色阳性细胞数、心肌梗死边缘区中Brdu与cTn I双染阳性细胞数均显著增高(P<0.01)。结论:apr-YTF能促使BMSCs动员入血、定向归巢并转化为心肌细胞,且优于rhG-CSF。

Objective： To observe the effect of Yangxin Tongmai Formula effective components recipe on bone marrow mesenchymal stem cells homing to the infarcted myocardium in rats. Methods： The acute myocardial infarction （AMI） rat model was devided into apr-YTF injection group, rhG-CSF injection group, PBS buffer solution group, then the rat heart function in vivo was examined; the ventricular scar area and capillary counts were detected; the CD34＋ and myocardial Brdu and cTnI double dyeing positive cells counts, and the content of factor VHI, VEGF, bFGF, Flk-1 in the myocardial tissue were detected; the peripheral blood CD34＋ cell counts was also detected, the differece between the three groups was analyzed. Results： （.]）Compared with PBS group, the LVSP, LVEDP, ＋dp/dtmax, -dp/dtmax were significantly improved in apr-YTF group and rhG-CSF group, the ventricular scar area percentage was decreased, the ventricular myocardial capillary counts, the CD34＋ cell counts in the myocardial infarction marginal region and peripheral blood, Brdu and cTn I double dyeing positive cells counts in the myocardial infarction marginal region, the expression of factor VII, Flk-1, VEGF, bFGF in the myocardial infarction marginal region in apr- YTF group and rhG-CSF group were significantly increased （P〈0.01, P〈0.05 ）. （~）Compared with rhG-CSF group, moreover, the LVSP, and the myocardial tissue CD34＋ cell counts, myocardium, and the Brdu and cTnI double dyeing positive cells counts in apr-YTF group was significantly increased （P 〈 0.01）. Conclusion： apr-YTF can promote BMSCs migrating to the blood, oriented homing and transferring to be mycardiocytes, which is better than rhG-CSF.