七味铁屑胶囊对NAFLD大鼠的疗效观察及ERS相关因子的影响

Therapeutic effect and the influence of the ERS regulated factors research of Qiwei Tiexie Capsule on non-alcoholic fatty liver rats

目的:观察七味铁屑胶囊对非酒精性脂肪性肝病(NAFLD)大鼠的疗效及初步探讨对ERS相关调控因子的影响。方法:Wistar大鼠60只,随机分为正常组(N组)、模型组(F组)、七味铁屑胶囊治疗组(Q组)、易善复胶囊阳性对照组(P组)。分别给予药物干预12周后,观察各组大鼠病理组织学特点,血清AST、ALT、TG、FFA和肝组织GRP78、SREBP-1c、Caspase-3、TNF-α蛋白表达水平。结果:12周时与F组比较,Q组和P组大鼠肝脏病理学改变明显减轻,其炎症程度、血清AST、ALT、TG、FFA水平及肝组织GRP78、SREBP-1c、Caspase-3、TNF-α表达量均明显降低(P<0.01);Q组和P组二者之间无统计学差异。结论:七味铁屑胶囊对单纯高脂饮食诱导的大鼠非酒精性脂肪性肝病疗效确切,可能通过下调GRP78水平,恢复内质网稳态,减轻脂质在肝内堆积,抑制炎症反应,从而改善肝脏脂肪变性及炎症。

Objective： To observe the effect of Qiwei Tiexie Capsule on non-alcoholic fatty liver disease （NAFLD）in rats, and preliminary discuss on its influence of the ERS regulated factors. Methods： Wistar rats 60, were randomly divided into normal group, model group, Qiwei Tiexie Capsule treatment group, and Polyene phosphatidylcholine Capsule positive control group. After the treatment of drugs for 12 weeks respectively, we observed the pathological and histological character, the levels of serum AST, ALT, TG, FFA and the protein expression levels of liver tissue GRP78, SREBP-lc, Caspase-3, and TNF-a. Results： Compared with model group, the pathological features of liver in Qiwei Tiexie Capsule treatment group and Polyenephos phatidylcholine capsule positive control group were significantly alleviated, and the degree of inflammation, levels of serum AST, ALT, TG, FFA and the expression of liver tissue GRP78, SREBP-lc, Caspase-3, TNF-c~ were significantly reduced （P〈0.01）; Qiwei Tiexie Capsule treatment group and Polyenephosphatidylcholine Capsule group had no statistical difference. Conclusion： Qiwei Tiexie Capsule has the exact effect on non-alcoholic fatty liver disease in simplex high fat diet rats, through reducing the expression of GRP78 protein in rat liver tissue, restoring the steady-state of endoplasmic reticulum, reducing the accumulation of lipid in the liver and inhibiting inflammatory, to moderate hepatic steatosis and inflammation degree.