苍耳内生真菌的分离鉴定及抗菌活性研究

Isolation,Identification and Antimicrobial Activity of Endophyte Fungi from Plant Xanthium sibiricum Patr

从植物苍耳中分离内生真菌,选出8株进行抑菌活性测定,有6株对番茄早疫病菌抑制率大于50%。菌株SC008对5种植物病原菌的抑制率均大于50%。对内生真菌SC008进行形态学观察及ITS序列鉴定,初步判定菌株SC008归属于黑孢霉属(Nigrospora Zim.)。碳氮源实验表明,SC008在以乳糖和蔗糖为碳源及以酵母提取物和牛肉膏为氮源的培养基上生长良好。

8 strains of endophytic fungi isolated from Plant Xanthium sibiricum Patr were tested with antimicrobial activity assay.6 strains of endophytic fungi showed high inhibitory effects on Alternaria solani,and the inhibition rates were all above 50%. Strain SC008 showed high inhibitory effects on plant pathogenic bacteria and fungi, the inhibition rates of 5 plant pathogenic bacteria and fungi were all above 50%. Strain SC008 was identified by morphological observation and ITS sequence analysis. The result indicated that strain SC008 was Nigrospora Zim. The carbon and nitrogen source assay showed that SC008 could grow fast on the culture medium which carbon source comes from lactose and sucrose, and nitrogen source come from yeast extract and beef extract.