固定化磷脂酶Lecitase~ Ultra催化合成果糖月桂酸单酯的研究

Synthesis of Monolauroyl Fructose Catalyzed by Immobilized Lecitase~ Ultra

利用固定化磷脂酶Lecitase Ultra在脱水叔戊醇中催化合成果糖月桂酸单酯。对该酶法合成果糖单酯反应体系进行了优化,考察了加酶量、酸糖摩尔比、分子筛添加量、糖浓度和时间等几个影响酯化反应的因素。结果表明:在4 mL反应体系中,固定化磷脂酶Lecitase Ultra添加量25 g/L、酸糖摩尔比4:1、果糖250 mmol/L、分子筛添加量100 g/L、43℃下反应48 h果糖月桂酸单酯的转化率达到69.45%。

Production of monolauroyl fructose catalyzed by immobilized Lecitase Ultra in anhydrous 2-methyl-2-butanol was investigated in this study. The process parameters was optimized as follows： reaction medium 4 mL, biocatalyst 25 g/L, glucose 250 mmol/L, laurie acid 1000 mmol/L, molecular sieves load 100 g/L, temperature 43 ℃. The maximum monolauroyl fructose conversion could reach 69.45% after 48 h.