大葱提取物对人胃癌细胞裸鼠皮下移植瘤生长的抑制作用研究

Study on inhibitory effect of Allium Fistulosum.L oil on the growth of transplantation tumor of human gastric cancer in subcutaneous of nude mice

目的观察大葱提取物大葱油对人胃癌MGC-803细胞裸鼠皮下移植瘤生长的抑制作用。方法建立人胃癌MGC-803细胞裸鼠皮下移植瘤模型,实验组腹腔注射10g/L大葱油注射液0.5 mL/次,对照组腹腔注射20 g/L吐温-80 0.5 mL/次,观察各组移植瘤生长情况,计算肿瘤生长抑制率及DNA图像分析DNA指数及平均DNA质量。结果实验组肿瘤生长抑制率为36.07%,肿瘤DNA指数和平均DNA质量下降。结论大葱提取物大葱油对人胃癌移植瘤生长具有抑制作用,抑制肿瘤细胞DNA指数及平均DNA质量可能是其抗肿瘤的机制。

Objective It is to observe the inhibitory effect of Allium Fistulosum. L oil （AFLO） on growth of transplanta- tion tumor of human gastric cancer in subcutaneous of nude mice. Methods The nude mice transplantation tumor model of hu- man gastric cancer in subcutaneous were made and divided into two groups, and then the animals were treated with 20 g/L tu- wen - 80 in control group and with 10 g/L AFLO in test group by peritoneal injection. The condition of growth of transplanta- tion tumor was observed in both groups, and the tumor growth inhibition ratio, neoplasm weight, tumor DNA index and average DNA contents were measured. Results The tumor growth inhibition ratio was 36.07% in test group, and the DNA index and the average DNA contents were decreased. Conclusion AFLO can inhibit the growth of transplantation tumor of human gastric cancer, the mechanism of inhibit tumor may be it can inhibit the DNA index and the average DNA contents.