PCR技术检测输卵管异位妊娠石蜡包埋组织支原体感染

Detection of mycoplasma infection in paraffin tissues of ectopic pregnancy by polymerase chain reaction

目的 :建立一种简便有效的石蜡包埋组织脱蜡及DNA提取方法 ,探讨支原体感染与输卵管异位妊娠的发生是否有关。方法 :应用套式聚合酶链反应技术 ,对 5 2例输卵管异位妊娠石蜡包埋组织 (病例组 )以及 2 0例输卵管结扎切除的石蜡包埋组织 (对照组 )进行PCR检测。结果 :病理组解脲支原体 (UU)与生殖支原体 (Mg)阳性率分别为 38.5 % ( 2 0 / 5 2 )及 5 .8% ( 3/ 5 2 ) ,阳性率为 44 .3 % ( 2 3/ 5 2 ) ;对照组UU与Mg阳性率分别为 15 % ( 3/ 2 0 )及 0 % ( 0 / 2 0 ) ,阳性率 15 % ( 3/ 2 0 )。两组支原体阳性率经卡方检验有显著性差别 (x2 =5 .35 ,P <0 .0 2 5 )。结论 :输卵管异位妊娠石蜡组织中存在支原体感染 ,并且支原体感染与输卵管异位妊娠的发生有一定关系。水浴脱蜡及NAI沉淀DNA方法比传统的二甲苯脱蜡以及苯酚抽提DNA有较多优点 :无毒无害易于操作 ,方法稳定重复性好 。

Objective:To establish a simple and useful method for waterbathing deparaffining and extraction of DNA.Try to find out whether the relation between mycoplasma infection and ectopic pregnancy exist or not.Method:Using nested polymerase chain reaction technique,to detect Ureaplasma urealyticum(UU) and Mycoplasma genitalium(Mg) in two groups of samples of paraffin embedded tissues which are 52 tubal pregnancies as cases and 20 fallectomic as controls.Results:The positive rate of UU and Mg in cases group was 38.5%(20/52)and 5.8%(3/52)respectively.The total was 44.3%(23/52).The positive rate of UU and Mg in control group was 15%(3/20)and 0%(0/20)respectively.Chisquare test indicates that mycoplasma infection in two groups were significantly differentia (x 2=5.35,P<0.025).Conclusion:The study pointed out that some relationship between mycoplasma infection and ectopic pregnancy exist.The method is advantaged than classical one because that was not toxic,not harmful to the people and easy to handle.Meanwhile the results are fine to repeat.So it is valuable to recommend.