培养法检测广州地区不同人群生殖支原体感染的研究

Study on infection of mycoplasma genitalium by culture and PCR in different population in Guangzhou area

目的 :研究广州地区不同人群生殖支原体 (Mg)感染情况。方法 :用SP 4培养基对 1997年 7月至 1997年 11月间本地区包括正常人群 10 7例、不洁性史妇女 10 7例、不洁性史 (冶游史 )男性 118例、不同医院的性病门诊患者 12 1例共 44 3例的泌尿生殖道拭子进行培养 ;采用Mg粘附因子基因序列合成的引物MgPa 1和MgPa 3 ,用多聚酶链反应方法在三次不同条件下对培养可疑阳性标本进行生殖支原体检测 ,并与阳性对照进行比较。结果 :培养标本中 ,正常人群全阴性 ,高危人群中72例可疑阳性。但经PCR检测 ,所有阳性标本均未能扩增出任何片断。结论 :目前

Objective:To study the prevalence of mycoplasma genitalium(Mg) in different populations in Guangzhou.Method:We cultivated Mg by SP4 medium in the urogenital tract swabs from 443 people in different populations including normal group (107),whoremongers(118),prostitutes(107) and patients (121)visiting STD department in the Second Municipal Hospital of Guangzhou and four other hospitals in this area between July 1997 to October 1997.By polymerase chain reaction in different conditions,we also detected Mg three times in all suspectable positive samples from culture based on the published nucleotide sequence of Mg adhesion protein gene MgPa1 and MgPa3.Result:No positive cultures in normal people,but there were 72 suspectable samples in high risk group.No positive amplification was found in suspectable positive samples by PCR.Conclusion:There were little Mg infections in Guangzhou at present.