摘要
用２．５％戊三醛作偶联剂，分别合成因清白蛋白－－氨苄西林钠抗原和卵白蛋白－－氨苄西林钠抗原。用牛血清白蛋白－－氨苄西林钠作免疫原，经兔背部多点注射，连续强化免疫五次（每次间隔１月）后，制备兔抗氨苄西林钠血清。用饱和硫酸铵法纯化抗血清，获抗氨苄西林钠ＩｇＧ，经酶联免疫测定最佳效价为１：１０００。用卵白蛋白－氨苄西林钠作包板抗了适浓度为８μｇ／Ｌ，４℃过夜，采用４１℃孵板反应温度。
A glutaraldehyde (2.5%) was acted as couple agent, beef seurm albume ampicillin antigen (BSA-Am) and ovalbumin-ampicillin antigen (OV-Am) were synthesized, respectively. Antiseurm against ampicillin was prepared with BSA-Am served as immunogen injected into back skin of rabbit with a number spot, antiseurm was pured by saturated amonia sulfate, With1: 1000 optimum titre (ELISA). OV-Am was acted as a coated -plate antigen, played 4℃ through night, with 8ug/L otimum concentration, determination of ampicillin content in m by ELISA was developed, with 41℃ reactive temperature. Concentration of detective ampiciliin showed 5－160μg/L, the minimum detective hat was 2.5μg/L, recovery with 100%±5 %. The antibody against ampicillin revealed affinity of 1.14×10~7, with 100K and 98% cross reaction for ampicillin and penicillin G, respectively.
出处
《中国卫生检验杂志》
2000年第1期10-12,共3页
Chinese Journal of Health Laboratory Technology
基金
安徽省教委资助!98JL183
