罗布麻外植体的筛选及愈伤组织诱导

Explants Selection and Callus Induction of Apocynum venetum L.

以罗布麻带芽茎段、茎段、幼根为外植体,探讨不同培养基、不同激素配比对罗布麻愈伤组织形成的影响。结果表明:适宜的罗布麻外植体为带芽茎段;适宜的培养基为MS培养基;适宜愈伤组织诱导的激素配比为6-BA0.6mg·L-1+NAA0.5mg·L-1,其愈伤组织诱导率达到100%。

The effect of different mediums and different hormone combinations on callus formation of Apocynum venetum L. was investigated using stems with a bud,stem and root of Apocynum venetum L. as explants. The results showed that the suitable explant was stem with a bud, the suitable medium for callus induction was： MS＋6-BA0.6 mg·L^-1 ＋NAA0.5 mg·L^-1 ,in which the callus induction rate reached 100%.