大鼠乳腺肿瘤细胞色素b基因序列分析

SEQUENCE ANALYSIS OF CYTOCHROME B GENE FROM RAT BREAST CANCER

该文用DNA自动测序方法 ,测定了大鼠乳腺癌组织、癌旁组织和正常组织的细胞色素b基因序列。结果表明 ,癌组织发生了nt190C→G ,nt2 6 3C→G和nt6 94T→C三处点突变。癌旁组织发生了nt6 95A→C突变。正常组织没有突变。

Using DNA auto-sequencing methods, we analised the sequences of mitochondrial cytochrome b gene from rat breast cancer tissues, paracacinoma tissues and normal tissues. The results showed that the cytochrome b gene from cancer tissues had three points mutations, namely, the C to G mutation at nt 190, the C to G mutation at nt 263 and the T to C mutation at nt 694. The cytochrome b gene from paracacinoma tissues had A to C mutation at nt 695. The cytochnme b gene from normal tissue had not mutation. The paracacinoma tissues were abnormal tissues in DNA molecular level.