反流性肾病患儿T淋巴细胞免疫活性的变化及其意义

THE CHANGE AND SIGNIFCANCE OF REFLUX INFANTILE NEPHROTIC T LYMPHOCYTIC IMMUNOCOMPETENCE

探讨细胞免疫在反返流性肾病 (RN)发病中的作用。方法 :通过流式细胞仪 (FCM ) ,应用单标和双标免疫荧光法 ,利用单克隆抗体测定 12例RN患儿外周血单个核细胞 (PBMC)表面膜抗原 ,从而分析RN患者T淋巴亚群及其活化情况。结果 :T淋巴细胞亚群CD+3、CD+4 与正常组相仿 ,CD+8增高 ,CD+4 CD+8比值低于正常 (P <0 .0 1) ;T细胞CD+2 5 表达高于正常 ,T细胞亚群活化表达CD+3CD+2 5 、CD+4 CD+2 5 、CD+8CD+2 5 分别较正常组高 (P <0 .0 1) ;活性化抗原CD+ 6 9,CD+ 71 均高于正常组 (P <0 .0 1) ;相关分析显示CD+ 4 CD+ 8比值与CD+ 8、CD+ 2 5 、CD+ 3、CD+ 2 5 均存在负相关 (P <0 .0 5 )。结论 :T细胞异常活化在RN发病机制中起重要作用 。

Objective: To investigate the role of cellular immunity in the pathogenesis of reflux nephropathy (RN). Methods: The flowcytometer was used to study the changes of T lymphocyte subsets from peripheral blood mononuclear cell of 12 cases RN patients through one or two colors immunofluorescence staining and a series of monoclonal antibodies. Results: There were no remarkable differences of the CD + 3, CD + 4 subsets between the patients and the normal, the CD + 8 was much higher than that of the normal, and CD + 4/CD + 8 ratio decreased ( P <0.01). The CD + 25 cells was significantly elevated, and the activation of T lymphocyte subpopulations CD + 3CD + 25 , CD + 4CD + 25 , CD + 8CD + 25 increased accordingly ( P <0.01). Lymphocyte ativation antigens CD + 69 , CD + 71 were significantly improved in RN ( P <0.01). The CD + 4/CD + 8 ratio has negative correlation with CD + 8, CD + 25 , CD + 3CD + 25 ( P <0.05). Conclusions: An aberrant activation of T lymphocytes might play an important role in the pathogenesis of RN, and disorder of T lymphocyte was relevant to the process of RN.