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C型产气荚膜梭菌cpe基因克隆与分析 被引量:1

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摘要 参照GeneBank上产气荚膜梭菌肠毒素(cpe)基因序列,设计合成一对特异性引物,采用PCR技术对产气荚膜梭菌C型分离株进行扩增,将扩增产物连接到pMDl8-Tgas载体,转化至大肠杆菌DH5a感受态细胞,提取质粒进行PCR和双酶切鉴定后测序,结果显示:产气荚膜梭菌C型分离株cpe基因全长960bp,可编码319个氨基酸;与参考株核苷酸同源性为99.4%~99.8%,氨基酸同源性为99.1%~997%。
作者 秦雯 文明
出处 《科技与生活》 2012年第14期172-173,共2页
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