TGFBI对胃癌细胞SGC-7901转移潜能影响的研究

TGFBI induces SGC-7901 adhesion,proliferation and migration

目的:探讨细胞外基质蛋白转化生长因子β诱导(TGFBI)对胃癌细胞SGC-7901转移潜能的影响。方法:牛血清白蛋白(BSA)、纤维粘连蛋白(FN)及细胞外基质蛋白TGFBI包被96孔板后,于其上培养SGC-7901细胞。MTT法检测其与TGFBI粘附能力及粘附后的增殖能力。同法包被Transwell小室滤膜下表面,检测其诱导SGC-7901细胞迁移的能力。结果:FN和TGFBI支持SGC-7901细胞粘附的能力明显高于BSA(P=0.023),FN和TGFBI之间差异无统计学意义,P>0.05;BSA、FN和TGFBI诱导SGC-7901细胞增殖的能力渐次增强,P=0.031;FN和TGFBI诱导SGC-7901细胞迁移的能力明显高于BSA(P=0.019),FN和TGFBI之间差异无统计学意义,P>0.05。结论:TGFBI可以增强胃癌细胞SGC-7901的转移潜能。

OBJECTIVE： To study the effect of transforming growth factor beta-induced （TGFBI） on the metastatic potential of SGC-7901. METHODS： The 96-well microculture plates were coated with BSA. Fibronectin （FN） and TGF- BI,on which SGC-7901 was cultured and the adhesion and proliferation was measured by MTT; the undersurface of the membrane of transwell plates was coated with BSA, fibronectin and TGFBI. The ability of migration was measured. RE- SULTS： FN and TGFBI could support SGC-7901 adhesion in a greater degree than that of BSA （P=0. 023）. There was no significant difference between FN and TGFBI （P〉0.05）. The ability of BSA, FN and TGFBI to induce SGC-7901 proliferation was increased in turn （P=0. 031）. FN and TGFBI could induce SGC-7901 migration in a greater degree than that of BSA （P=0. 019）. There was no significant differences between FN and TGFBI （P〉0.05）. CONCLUSION： TG- FBI can improve the metastatic potential of SGC-7901.