胃癌组织ING1抑癌基因表达及其生物学意义探讨

Expression and significance of ING1 gene in gastric cancer

目的:探讨生长抑制因子1(ING1)基因表达及其与胃癌临床病理特征的关系。方法:荧光定量PCR技术和免疫印迹技术检测70例胃腺癌组织及癌旁对照胃黏膜组织中ING1基因的表达水平,并分析其与胃癌患者临床特征的关系。结果:70例癌组织中有57例(81.43%)ING1基因mRNA表达明显下调,其中4例组织为ING1表达缺失,与癌旁正常胃黏膜比较差异有统计学意义,P<0.01。ING1基因表达与胃癌患者的性别无明显相关性,与胃癌的分化程度、肿瘤累及深度以及淋巴结转移有关。结论:ING1在人胃癌细胞中表达下调,其表达水平与分化程度、肿瘤累及深度以及淋巴结转移有关。

OBJECTIVE： To explore the clinicopathological significance of expression of ING1 gene in gastric cancer. METHODS： The expression levels of INO1 in 70 gastric cancer and normal controls were detected by real-time PCR, Western blot. Cell apoptosis was evaluated by TUNEL and flow cytometry. RESULTS： In comparison with normal gastric tissues,INGl gene expression was obviously down-regulated in （81.43 ~, 57/70） gastric cancer tissues, there was a significant difference be- tween two groups （P^0.01）. The expression level in cancer patients was associated with the histological differentiation, invasive depth and lymphatic metastasis and there was no association with the gender. CONCLUSION： ING1 down-regulated in gastric cancer may play an important role in the pathogenesis and consequence of gastric cancer.