宫颈病变卡波济肉瘤相关疱疹病毒感染状况分析

Analysis of Kaposi's Sarcoma-associated Herpesvirus Infection Status in Females with Cervical Lesions

目的:了解宫颈病变卡波济肉瘤相关疱疹病毒(KSHV)感染状况,探讨KSHV感染与宫颈病变的关系方法:选择组织学确诊为宫颈上皮内瘤变(CIN)和宫颈癌(SCC)的病例440例作为研究组,同区域、同期就诊或体检并排除有宫颈病变的女性380例为对照组,分别采集血液和宫颈脱落细胞标本,提取DNA,巢式PCR检测KSHV ORF26,核酸电泳观察结果结果:研究组血液样品KSHV检出率12.9%(53/412),对照组检出率6.5%(23/354),两组差异有统计学意义(P<0.01),但宫颈脱落细胞样品中未能检出KSHV;对研究组作分层分析,KSHV检出率随年龄增加、宫颈病变程度加重而升高,差异有统计学意义(P<0.05)结论:宫颈病变女性KSHV检出率高于正常女性,感染率随宫颈病变程度加重而升高。

Objective： To aimed to analyze the infection status of female patients with Kaposi＇s sarcoma-associated herpesvirus （KSHV） and its relationship with the occurrence of cervical lesions. Methods： For the study group, 440 histological cases with con- firmed cervical intraepithelial neoplasia （CIN） and cervical squamous cell cancer （SCC） were randomly selected. Simultaneously, 380 cases without cervical lesions in the same region were physically examined as the control group. Blood and cervical cell samples were collected for DNA extraction, and the KSHV ORF26 was detected using nested PCR and gel electrophoresis. Results： The KSHV detec- tion rate of blood samples in the study group was 12.9% （53/412）, whereas that in the control group was 6.5% （23/354）. The difference between the two groups was statistically significant （P 〈 0.01）, but KSHV could not be detected in the cervical cell samples. Stratified analysis was applied for the study group, and the detection rate of KSHV was found to significantly increase with the aggravation of cer- vical lesions and with increasing age （P 〈 0.05）. Conclusion： The KSHV detection rate in females with cervical lesions is higher than in normal females. Thus, KSHV can potentially strengthen the monitoring of cervical lesions.