骨髓干细胞移植加动员在重症急性胰腺炎大鼠小肠黏膜细胞凋亡中的调节作用

Effect of Bone Marrow Mesenchymal Stem Cells Transplantation Combined with Mobilization on Cell Apoptosis of Intestinal Mucosa in Rats with Severe Acute Pancreatitis

目的探讨骨髓间充质干细胞对重症急性胰腺炎大鼠肠黏膜细胞凋亡的保护机制。方法 240只SAP大鼠分为对照组、SAP组、干细胞组、动员组及联合组,各组再平分为术后12、24、48、72h组。干细胞动员组用G-CSF液动员3天,干细胞组一次性静脉注射MSC液,联合组动员加注射MSC。记录大鼠的病死率,肠黏膜上皮细胞的Chiu评分,凋亡蛋白Bax、Bcl-2基因水平,细胞凋亡指数AI,比较联合治疗效果。结果各组72h病死率差异不显著(P>0.05);治疗组肠黏膜上皮损伤程度较轻,联合组48h后Chiu病理评分与单独治疗组比较差异显著(P<0.05);治疗组AI及Bax蛋白水平随时间延长较SAP组下调,Bcl-2蛋白水平上调,联合组与干细胞组、动员组比较差异显著(P<0.05),而动员组与干细胞组之间并无显著差异(P>0.05)。结论 MSC静脉移植与动员能抑制肠黏膜上皮细胞过度凋亡,维持肠黏膜屏障完整性,能有效减轻重症急性胰腺炎小肠黏膜的损伤,联合治疗效果优于单独治疗。

Objective To discuss the protective mechanism of MSCs transplantation and mobilization in acute intestinal mucosa inju- ry with SAP. Methods Totally 240 SAP rats were randomly divided into comparing group, SAP group, MSC group, mobilization group and combination group. Rats in each group were subdivided into 12h, 24h, 48h and 72h groups. Rats in mobilization group were injected G - CSF 401xg/kg for 3 days,in MSC group were injected I. 2ml MSC,in combination group both were injected MSC and G - CSF. The mortality rate, Chiu pathological scores, levels of Bax, Bcl -2 proteins and apoptosis indexes of intestinal mucosa epithelium cells were recorded. The difference of each group was compared. Results The mortality rate of each group showed no difference at 72h（ P 〉 0.05 ）. The pathological injurys of treat groups were relieved. Chiu scores in combination group at 48h was different from single groups. The apop- tosis indexes and Bax protein level in treated groups were lower than those in SAP group. Bcl -2 protein level was also higher. The differ- ence between combination treatment group and sigle treatment group was obvious （ P 〈 0.05 ） , but no difference between two single treat groups was found （P 〉 0.05）. Conclusion MSC transplantation and mobilization can inhibit cell apoptosis, maintain intestinal mucosa epithelium cells barrier. Combination group is superior than sigle groups.