Per2和cyclin D1在非小细胞肺癌中的表达

Expression of Circadian Gene Per2 and Cyclin D1 in NSCLC

目的研究生物钟基因Per2和细胞周期蛋白D1(cyclin D1)在非小细胞肺癌中的表达,探讨其临床意义。方法采用免疫组织化学染色法检测60例非小细胞肺癌组织和20例正常肺组织中Per2和cyclin D1的表达并分析其表达与临床病理特征的关系。结果在非小细胞肺癌和正常肺组织中Per2的阳性表达率分别为71.7%和95.0%(P<0.05),而cyclin D1的阳性表达率分别为63.3%和15.0%(P<0.01)。非小细胞肺癌中Per2的表达缺失与分化程度及TMN分期相关(P<0.05),而cy-clin D1的阳性表达和TNM分期及淋巴结转移相关(P<0.05)。结论 Per2在非小细胞肺癌中表达降低,而cyclin D1则呈高表达,两者可以作为判断非小细胞肺癌发生、发展和预后的指标。

Objective To detect the expression of Per2 and cyclin D l in non - small - cell lung cancer （NSCLC） , and analyze their relation to clinical pathological data. Methods The expression of Per2 and cyclin D1 was measured in 60 NSCLC and 20 normal lung tissue by immunohistochemistry assay, then we analyzed the relation with clinical pathological parameters. Results The positive ex- pression rates of Per2 in NSCLC and in normal lung tissue were 71.7% and 95.0% （P 〈 0.05 ） , while the positive expression rates of cy-clin D1 were 63.3% and 15.0% （ P 〈 0.01 ）. The negative expression of Per2 in NSCLC was correlated with pathological differentiation or TNM stage（ P 〈 0.05 ） ,while the positive expression of cyclin D1 in NSCLC was correlated with TNM stage or lymphatic metastasis （P 〈 0.05 ）. Conclusion The expression of Per2 in NSCLC was decreased, while the expression of cyelin D1 was increased. The detection of them could be an important marker to evaluate the malignancy degree and the prognosis of NSCLC.