小鼠毛囊来源间充质干细胞体外诱导成软骨的细胞标记物

Cell surface markers during chondrogenic indution of mesenchymal stem cells derived from mouse hair follicle in vitro

背景:小鼠毛囊来源的间充质干细胞有很强的多向诱导分化能力,但少有研究关注其在诱导分化为软骨细胞过程中的细胞表面标记物。目的:观察小鼠毛囊来源的间充质干细胞分化为软骨细胞过程中的形态、细胞表面标记物和硫酸黏多糖含量的变化。方法:体外分离培养ICR新生小鼠皮肤干细胞,用成软骨细胞诱导液诱导培养7,14d观察各项指标。结果与结论:经成软骨细胞诱导液处理后,小鼠毛囊来源的间充质干细胞中CD44(+)细胞数量无明显增加,硫酸黏多糖含量无变化,CD54(+)细胞和CD166(+)细胞则显著增加,两者硫酸黏多糖含量亦明显升高。表明小鼠毛囊来源的间充质干细胞能诱导形成软骨样细胞;CD44不能作为小鼠毛囊来源的间充质干细胞向软骨细胞分化的细胞表面标记物;CD54和CD166以及硫酸黏多糖可作为对小鼠毛囊来源的间充质干细胞向软骨细胞分化的监测指标。

BACKGROUND：Mesenchymal stem cells derived from mouse hair follicle possess multipotential differentiation ability. There have been few reports describing cell surface marker during chondrogenic induction process of mesenchymal stem cells derived from mouse hair follicle in vitro. OBJECTIVE：To investigate cell morphology, cell surface markers and sulfate glycosaminoglycan level during chondrogenic induction of mesenchymal stem cells derived mouse hair follicle. METHODS：IRC newborn mouse skin stem cells were in vitro isolated and cultured. Then cells were cultured with chondrogenic culture medium for 7 and 14 days for determination of indices. RESULTS AND CONCLUSION：After treated with chondrogenic medium, the counts of CD44＋ cells did not increase dramatically and the level of sulfate glycosaminoglycan was not changed, but the counts of CD54＋ cells and CD166＋ cells were significantly increased and the levels of corresponding sulfate glycosaminoglycans showed the same increasing tendency. These results suggest that mesenchymal stem cells derived from mouse hair follicle could differentiate into chondrocyte-like cells under the induction of medium; CD44 could not be used as the cell surface marker of chondrogenic differentiation of mesenchymal stem cells derived from mouse hair follicle, while CD54 and CD166 as well as corresponding sulfate glycosaminoglycans are competent for cell surface from marker of chondrogenic differentiation of mesenchymal stem cells derived from mouse hair follicle.