毛囊干细胞对坐骨神经损伤修复的影响

Hair follicle stem cells in repair of sciatic nerve injury

背景:毛囊干细胞具有多分化潜能,可分化成神经细胞,极有希望成为治疗周围神经损伤的种子细胞。目的:观察毛囊干细胞对坐骨神经损伤修复的影响。方法:体外分离培养SD大鼠乳鼠胡须处的毛囊干细胞,经鉴定备用。36只SD大鼠随机分为实验组和对照组,建立坐骨神经损伤模型后,实验组于坐骨神经损伤处的上方注入浓度约106L-1的毛囊干细胞50μL,对照组注射等量的磷酸盐缓冲液。结果与结论:各组坐骨神经功能指数均随观察时间进行性增加,其中实验组大鼠神经功能恢复早于对照组;免疫组织化学检测移植后的毛囊干细胞大量存活并分化成神经细胞。结果提示毛囊干细胞能够有效的促进损伤的坐骨神经修复。

BACKGROUND：Hair follicle stem cells are multipotent which can differentiate into neurons and repair injured peripheral nerves. OBJECTIVE：To investigate the effects of hair follicle stem cells on repair of injured sciatic nerve. METHODS：Hair follicle stem cells were isolated and cultured in vitro. Thirty-six healthy rats were selected to prepare sciatic nerve crush injury models with clamping method. Subsequently, rats were randomly divided into a transplantation group and a control group, with eighteen rats in each group. Rats in the transplantation group underwent transplantation of 50 μL of hair follicle stem cells at a concentration of 106/L into nerve injured sites. At the same time, equal amount of PBS was injected into the rats in the control group. RESULTS AND CONCLUSION：Nestin, CK19, anti-integrin beta 1 positive cells could be found at injured nerve in the transplantation group 4 and 8 weeks after surgery. Sciatic nerve function indices in the transplantation group superiorly recovered to those in the control group 3-6 weeks after surgery. Hair follicle stem cells transplanted into injured nerve tissue promote the recovery of injured sciatic nerve.