CD133和CD34在肝细胞癌血管生成拟态形成中的表达及意义

Expression and significance of CD34 and CD133 in vasculogenic mimicry of hepatocellular carcinoma

背景:在恶性肿瘤中血管生成拟态的形成过程与肿瘤干细胞有密切联系。目的:分析肝癌干细胞标志物CD133和CD34在肝细胞癌血管生成拟态形成中的表达及意义。方法:建立肝癌细胞HCC97H、SMMC7721和正常肝细胞L02三维培养体系,结合激光捕获显微切割技术分离形成血管生成拟态的肝癌细胞,分别利用RT-PCR和Western blot技术检测CD133和CD34表达水平。结果与结论:三维培养条件下,肝癌细胞HCC97H细胞形成血管生成拟态,肝癌细胞SMMC7721以及正常肝细胞L02未形成血管生成拟态。形成血管生成拟态的肝癌细胞HCC97H中CD133、CD34在mRNA及蛋白表达水平上均高于未形成血管生成拟态的肝癌细胞SMMC7721和正常肝细胞L02(P<0.05)。表明高侵袭性肝癌细胞在三维培养下形成血管生成拟态,而低侵袭性肝癌细胞及正常肝细胞不能形成血管生成拟态;肝癌细胞形成血管生成拟态的过程中与表达肝癌干细胞有关。

BACKGROUND：Formation of vasculogenic mimicry in malignant tumor is closely linked to the cancer stem cells. OBJECTIVE：To analyze the expression and significance of CD34 and CD133 in vasculogenic mimicry of hepatocellular carcinoma. METHODS：We established three-dimensional cell cultures in several cell lines, such as HCC97H cell, SMMC7721 cell and normal L02 cell. Liver cancer cells which formed vasculogenic mimicry were isolated by the laser capture microdissection, and the expressions of CD133 and CD34 were analyzed by semi-quantitative reverse transcription- polymerase chain reaction and Western blot subsequently. RESULTS AND CONCLUSION：HCC97H cell had the ability to form vasculogenic mimicry in three-dimensional cell cultures, while SMMC7721 and L02 cell did not had the ability. The expressions of CD133 and CD34 in HCC97H cell were significantly higher than those in SMMC7721 cell and L02 cell （P 0.05）. Highly aggressive liver cancer cell has the ability to form vasculogenic mimicry in three-dimensional cell cultures, while poorly aggressive liver cancer cell and normal liver cell do not have the ability. Liver cancer stem cells impact the formation of vasculogenic mimicry in hepatocellular carcinoma.