成人关节软骨细胞增殖与人参皂甙Rg1的促进作用

Ginsenoside Rg1 promotes the proliferation of cultured adult human articular chondrocytes in vitro

背景:人参总皂甙的主要有效成分为人参皂甙Rg1,有促进细胞增殖、抗衰老、抗细胞凋亡、抗炎及抗自由基等作用,但以往实验对象多为鼠、兔等动物的关节软骨细胞。目的:体外培养成人软骨细胞,观察人参皂甙Rg1对体外培养的成人关节软骨细胞增殖的促进作用。方法:实验分2组,Rg1组使用含质量浓度为40mg/L人参皂甙Rg1的DMEM培养液培养,对照组使用未加人参皂甙的DMEM培养液培养,每日应用倒置相差显微镜观察细胞生长情况至第7天,并进行细胞计数。同时培养至第6天检测软骨细胞中Ⅱ型胶原的表达。结果与结论:对照组自培养第2天起细胞进入对数增殖期,到第7天细胞数约为接种时的8倍。Rg1组自培养第2天起细胞增殖能力较对照组增强(P<0.05),到第7天细胞数约为接种时的18倍(P<0.05)。免疫组织化学SABC法检测结果显示,Rg1组与对照组细胞胞浆均可见Ⅱ型胶原表达,两组差异无显著性意义(P>0.05)。说明人参皂甙Rg1可促进成人软骨细胞的增殖。

BACKGROUND： The main active ingredient of ginseng saponins is ginsenoside Rgl that has the advantages of promoting cell proliferation, anti-aging, anti-apoptotic, anti-inflammatory and anti-free radical. However, the previous experimental objects are often the articular chondrocytes of mice, rabbits and other animals. OBJECTIVE： To observe the effect of ginsenoside Rgl on the proliferation of in vitro cultured adult articular chondrocytes. METHODS： The adult articular chondrocytes were cultured in vitro and divided into two groups： experimental group and control group. The experimental group was cultured with DMEM medium containing 40 mg/L ginsenoside Rgl, and the control group was cultured only with DMEM culture medium. After enzyme digestion, blood count plate was used to count cells, and cell growth curve plotted daily to the 7 day was observed by inverted phase contrast microscope. Type Ⅱ collagen expression in chondrocytes was detected on the 6th day. RESULTS AND CONCLUSION： After 2 days of incubation, ceLls in the control group entered the logarithmic growth phase, and on the 7th day, the ceils number was about eight times that on the inoculation day. The cell proliferation capacity of the experimental group enhanced after 2 days, which was higher than that of the control group （P 〈 0.05）, and on the 7 th day, the number of cells was about 18 times that on the inoculation day （P 〈 0.05）. Immunohistochemical SABC results showed that both groups had type Ⅱ collagen expression in the cytoplasm, and there was no significant difference between the two groups （P 〉 0.05）. Ginsenoside Rgl could promote the proliferation of articular chondrocytes.