子宫缺血再灌注损伤大鼠模型的建立

Establishment of uterine ischemia-reperfusion injury models in experimental rats

背景:子宫缺血再灌注损伤可导致能量代谢障碍、大量自由基产生及细胞凋亡等。目的:建立一种简易、实用、可靠的子宫缺血再灌注损伤大鼠模型。方法:将50只健康Wistar雌性大鼠随机等分为5组。子宫缺血组(共3组)开腹后采用线栓法分别阻断大鼠子宫动脉45min、阻断大鼠腹腔动脉30min或阻断大鼠腹腔动脉45min进行子宫缺血处理;子宫缺血再灌注组开腹后采用线栓法阻断大鼠腹腔动脉30min再灌注60min;假手术组不阻断子宫供血动脉。结果与结论:子宫动脉纤细,结扎过紧,子宫动脉常常断裂,结扎过松,不能够充分阻断血液,不适宜建立子宫缺血再灌注损伤模型。苏木精-伊红染色显示,阻断大鼠腹腔动脉30min后子宫细胞及间质肿胀,肌纤维排列尚整齐;阻断大鼠腹腔动脉45min及子宫缺血再灌注组的子宫细胞及间质明显水肿,渗出增加,肌纤维排列紊乱,有大量中性粒细胞浸润。分光光度计检测显示,阻断大鼠腹腔动脉45min及子宫缺血再灌注组子宫丙二醛水平升高最明显(P<0.01)。提示采用线栓法结扎腹腔动脉30min后进行再灌注可建立大鼠子宫缺血再灌注损伤模型。

BACKGROUND： Uterine ischemia-reperfusion injury can lead to energy metabolism dysfunction, formation of a large number of free radicals and apoptosis, etc. OBJECTIVE： To establish a simple, functional and reliable rat model of uterine ischemia-reperfusion injury METHODS： Fifty healthy Wistar rats were randomly divided into five groups. The uterine ischemia group （three groups）： uterus artery of the models in the uterine ischemia group 1 was ligatured for45 minutes by suture method after laparotomy celiac artery of the models in uterine ischemia group 2 was ligatured for 30 minutes and the celiac artery of the models in uterine ischemia group 3 was ligatured for 45 minutes. In uterine ischemia-reperfusion group, the celiac artery was ligatured for 45 minutes by suture method after laparotomy and then reperfusion for 60 minutes. In sham-operation group, the uterine blood supply artery was not blocked. RESULTS AND CONCLUSION： The uterine artery slim was due to the tight ligation, uterine artery rupture was due to the loose ligation which could not fully block the blood, so these two methods were not suitable to establish a uterine ischemia-reperfusion injury model. Hematoxylin-eosin staining showed that uterine cells and stroma was swelling, and neatly arranged muscle fibers could be seen after the celiac artery was ligatured for 30 minutes; in uterine ischemia-reperfusion group and celiac artery ligation for 45 minutes group, significant edema could be seen in the uterine cells and stroma cells, the exudation was increased and the muscle fiber was arranged in disorder, a massive neutrophil infiltration could be seen. Spectrophotometer detection showed that the content of uterine malondialdehyde was significantly increased in uterine ischemia-reperfusion group and celiac artery ligation for 45 minutes group （P 〈 0.01 ）. It indicated that the rat uterine ischemia-reperfusion injury model could be established through reperfusion after the celiac artery was ligatured for 30 minutes by suture method.