骨肉瘤动物模型的建立

An animal model of osteosarcoma

背景:为进一步研究骨肉瘤的发病机制,国内外学者通过不同方法建立了多种骨肉瘤动物模型。目的:对骨肉瘤模型的建立方法以及这些模型的特点进行综述。方法:应用计算机检索国内外数据库中关于骨肉瘤动物模型的文章,最终选择17篇文献进行综述。结果与结论:骨肉瘤模型主要分为实验性动物模型和自发性动物模型,目前大多骨肉瘤模型多属于实验性动物模型,其建立方法主要包括放射性核素诱导、化学物质诱导以及生物致瘤因素诱导、异体移植等。对肺高转移细胞进行筛选并建立相应模型的方法主要有3种,体内筛选、体外筛选、体内外筛选共同建立模型。不论是建立骨肉瘤细胞系还是动物模型,一般都需要体外重复传代培养,体内多次筛选等处理,不同学者虽然建立方法大致相同,但处理细节过程不同,这样建立的动物模型稳定性到底如何,目前没有统一的认识和标准。

BACKGROUND： Scholars have established animal models of osteosarcoma through a variety of different methods to further study the pathogenesis of osteosarcoma. OBJECTIVE： To review the establishing methods of osteosarcoma models, animal models of metastatic pulmonary osteosarcoma as well as the characteristics of these models. METHODS： A computer-based search of databases about animal models of osteosarcoma was performed, and finally 17 articles were included in result analysis. RESULTS AND CONCLUSION： Osteosarcoma models are classified into experimental animal models and spontaneous animal models. Current models of osteosarcoma mostly belong to experimental animal models. Methods to establish experimental animal models include radionuclides induction, chemical induction, bio-oncogenic factor induction, allograft and so on. There are three methods to screen metastatic pulmonary osteosarcoma and establish appropriate models： in vivo screening, in vitro screening, in vivo and in vitro screening. Establishment of osteosarcoma cell lines or animal models generally requires repeated in vitro subculture and in vivo screening. Scholars use the same method for model establishment, but their details of the establishing process are different. Therefore, there is no uniform understanding and standards for the stability of established animal models.