成骨生长肽促进钛表面大鼠成骨细胞的增殖分化

Osteoblastic growth peptides induce the proliferation and differentiation of rat osteoblast-like cells on titanium surface in vitro

背景:成骨生长肽具有促进多种基质细胞增殖活性,成骨活性,免疫原性低,自身调节极其敏感,提取制作工艺简单等众多优点。目的:体外观察成骨生长肽对大鼠颅盖骨来源成骨细胞在钛金属表面增殖分化的影响。方法:将体外培养的新生SD大鼠颅盖骨成骨细胞以5×107L-1细胞浓度接种于6孔板中的纯钛试件表面,分别加入0(空白对照),10-10,10-9,10-8,10-7mol/L的成骨生长肽,干预1,3,5,7,9d后应用MTT法检测纯钛试件表面成骨细胞的增殖活性,应用酶联免疫法检测成骨细胞内碱性磷酸酶活性。结果与结论:与空白对照组比较,各浓度成骨生长肽组纯钛试件表面成骨细胞增殖活跃(P<0.05),且最佳作用浓度为10-9mol/L(P<0.05);各浓度成骨生长肽组细胞内碱性磷酸酶活性增强(P<0.05),且最佳作用浓度为10-8mol/L(P<0.05)。表明成骨生长肽可促进钛片表面成骨细胞的增殖活性,增强细胞内碱性磷酸酶活性。

BACKGROUND： Osteogenic growth peptides have many advantages, such as low immunogenicity, self regulating sensitivity and simple extraction and making process, which can promote the proliferation activity and osteogenic activity of a variety of stromal cells. OBJECTIVE： To observe the effect of osteogenic growth peptides on proliferation and differentiation of rat cranium derived osteoblasts on titanium surface in vitro. METHODS： Sprague Dawley rat cranium derived osteoblasts at a concentration of 5×10^7/L were cultured in vitro and located on the surface of titanium disc in the 6-well plate, then the osteogenic growth peptide at concentrations of 0 （blank control）, 10^-4, 10^-9, 10^-8 and 10^-7 mol/L was added. The proliferative activity was examined by MTT, and alkaline phosphatase activity was examined by enzyme-linked immunosorbent assay individually after induced for 1,3, 5, 7 and 9 days. RESULTS AND CONCLUSION： Compared with the blank control group, the proliferation of the osteoblasts on the surface of titanium in different osteogenic growth peptide groups was increased （P 〈 0.05）, and the best concentration of osteogenic growth peptide was 10^-9 mol/L （P 〈 0.05）; the alkaline phosphatase activity in different osteogenic growth peptide groups was increased （P 〈 0.05）, and the best concentration of osteogenic growth peptide was 10^-8 mol/L （P 〈 0.05）. Our results demonstrate that osteogenic growth peptides can stimulate proliferative activity and alkaline phosphatase activity of rat osteoblasts located on titanium surface in vitro.