温敏性壳聚糖水凝胶复合细胞因子修复兔关节软骨缺损

Thermosensitive chitosan-based hydrogel containing cytokines for rabbit full-thickness articular cartilage defects

背景:单独以细胞因子修复软骨缺损时局部很难保持有效浓度,且维持时间非常有限。目的:观察负载基质细胞衍生因子1β、转化生长因子β1温敏性壳聚糖水凝胶修复兔关节软骨缺损的可行性。方法:通过京尼平的交联作用制备温敏性壳聚糖水凝胶,并负载基质细胞衍生因子1β、转化生长因子β1。取24只兔制作双侧膝关节软骨全层缺损模型,随机分为3组:结合组软骨下骨钻孔后以负载基质细胞衍生因子1β、转化生长因子β1的温敏性壳聚糖水凝胶充填软骨缺损,钻孔组行软骨下骨钻孔,对照组不做任何处理。结果与结论:温敏性壳聚糖水凝胶在37℃凝胶时间为3min,结构致密,为多孔三维支架,能缓慢释放基质细胞衍生因子1β、转化生长因子β1。结合组软骨修复在组织细胞形态、超微结构、Ⅱ型胶原含量等方面均明显优于钻孔组和对照组(P<0.05)。说明负载基质细胞衍生因子1β、转化生长因子β1温敏性壳聚糖水凝胶结合软骨下骨钻孔能有效修复兔膝关节软骨全层缺损。

BACKGROUND： Cytokines in the repair of cartilage defects are difficult to maintain their effective local concentration, and the maintenance of time is very limited. OBJECTIVE： To investigate the feasibility of repairing rabbit full-thickness articular cartilage defects with thermosensitive chitosan-based hydrogel containing stromal cell derived factor 1 β and transforming growth factor 1β. METHODS： The thermosensitive chitosan-based hydrogel containing stromal cell derived factor 1β and transforming growth factor 1β was prepared by genipin crosslinking. Experimental full-thickness articular cartilage defects models were created in the condyles of the bilateral femurs of 24 rabbits. The 48 defects were randomly divided into three groups. In the combination group, the thermosensitive chitosan-based hydrogel containing stromal cell derived factor 1β and transforming growth factor 1β was used to fill the defects after subchondral bone drilling; in the drilling group, only subchondral bone drilling was performed; and the control group just left alone as controls. RESULTS AND CONCLUSION： The thermosensitive chitosan-based hydrogel could be gelled within 3 minutes at 37 ℃. The hydrogel as multi-hole tree-dimensional scaffold with a dense structure and could sustain release stromal cell derived factor 1β and transforming growth factor 61. The combination group gave better results than drilling group and the control group in the aspects of cell morphology, type-Ⅱ collagen content and ultrastructure （P 〈 0.05）. It shows the thermosensitive chitosan-based hydrogel containing stromal cell derived factor 1β and transforming growth factor β1 combined with subchondral bone drilling can repair rabbit full-thickness articular cartilage defects effectively.