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人OCTN2原核表达载体PGEX-2T-OCTN2的构建及意义

Construction and Significanc of the vector PGEX-2T-OCTN2
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摘要 目的选取并克隆人特异性OCTN2基因片段,构建原核表达载体PGEX-2T-OCTN2。方法应用RT-PCR方法从人附睾组织中克隆特异性人OCTN2基因片段,通过双酶切方法把OCTN2基因片段克隆到PGEX-2T载体,再经双酶切和测序鉴定。结果获取并克隆特异性人OCTN2的基因片段,构建原核表达载体PGEX-2T-OCTN2。结论成功构建原核表达载体PGEX-2T-OCTN2,为进一步研究附睾肉碱转运机制奠定前期基础。 Objective To clone specific OCTN2 gene segments from human epididymis and construct the vector PGEX-2T-OCTN2. Methods The specific gene segments of OCTN2 was amplified from human epididymis by RT-PC.The PCR product was cloned into PGEX-2T vector by ECoR I and BamH I sites,and confirmed by DNA sequencing. Results The specific segmental coding region of OCTN2 was cloned and the vector PGEX-2T-OCTN2 was constructed successfully. Conclusion The cloning of OCTN2 and the construction of the vector PGEX-2T-OCTN2 would help to explore the mechanism of camitine transport in the epididymis furthermore.
作者 龚东明
机构地区 上海市浦东新区中医医院外科
出处 《中国医药指南》 2012年第23期402-404,共3页 Guide of China Medicine
关键词 OCTN2 PGEX-2T 附睾 肉碱 OCTN2 PGEX-2T Epididymis Camitine
分类号 R596 [医药卫生—内科学]
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参考文献5

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二级参考文献12

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中国医药指南
2012年 第23期

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