广西GAP基地穿心莲中脱水穿心莲内酯的含量及质量标准探讨

The content of dehydro-andrographolide in Andrographis paniculata(Burm.f.)Nees From GAP Bases in Guangxi and Have a discussion in quality standards

目的考察广西GAP基地穿心莲药材中脱水穿心莲内酯的含量,为提高其质量标准提供参考。方法采用HPLC法,色谱柱为Diamonsil-ODS C18(250 mm×4.6 mm,5μ);流动相为甲醇-水(65:35);流速为0.8 mL/min;检测波长为254 nm,柱温:25℃。结果广西GAP基地所产穿心莲药材中脱水穿心莲内酯含量相对稳定。结论实施药材GAP规范化种植是稳定中药有效成分、提高药材质量的有效途径。

Objective To investigate the content of dehydro-andrographolide in Andrographis paniculata（ Burm. f. ） Nees from GAP bases in Guangxi, and to set up a reference to improve its quality standards. Methods Diamonsil - ODS C18 （5μ 250mm×4.6mm） columu was adopted, the mobile phase consisted of methanol-water （65：35） eluted at the flow rate of 0.8 mL/min, the detection wave length was 254 nm; the column temperature was 25 ℃ ; Results The content of dehydro - andrographolide in Andrographis paniculata（ Burm. f. ） Nees from GAP bases were little discrepancy in Guangxi ; Conclusions GAP implantation was a positive way to insure that the active constituents of TCM to be stabilize.