我国32种鸟类DNA条形码分析

A Study of DNA Barcoding on 32 Species of Bird in China

DNA条形码是一种分子分类方法,近年来在物种鉴定方面得到迅速的发展和应用。本研究分析了我国27属32种鸟类(61只)的线粒体细胞色素c氧化酶亚基Ⅰ(COⅠ)基因的条形码片段,分别用阈值法、聚类法和诊断核苷酸进行了分析,探究DNA条形码鉴定我国鸟类的准确性。结果显示,种内COⅠ序列变异很小,种间存在较多的变异位点,种间的遗传距离显著大于种内的遗传距离,DNA条形码序列能够鉴定所有鸟类。

DNA barcoding has been proposed as a promising tool for the rapid species identification in a wide range of ani- mal taxa. In this study, the -648 bp mitoehondrial cytochrome e oxidase subunit Ⅰ （CO Ⅰ ） gene of 61 individuals from 32 bird species was analyzed. By applying three methods （ distance based method, monophyly-based method, and diagnostic characters）, our result shows that CO Ⅰ is able to distinguish all species studied. The present study validated the effective- ness of barcoding for the identification of birds.