神经生长导向因子Slit2在鸡胚脊髓发育中的表达

Expression of neuronal guidance factor Slit2 in the spinal cord of chick embryonic development

目的 观察神经生长导向因子Slit2在鸡胚神经管和脊髓不同发育时期的表达变化。方法 用免疫组织化学方法检测Slit2蛋白在鸡胚原肠期(HH6-HH10)神经管和第3d-17d(E3-E17期)脊髓中的表达和分布情况。结果 Slit2蛋白在鸡胚神经管和脊髓不同发育时期均有阳性表达,在脊髓中线结构区呈优势表达,在第9d(E9期)脊髓中线底板处表达最明显,第11d后Slit2蛋白阳性表达逐渐减弱并呈散在分布。结论 神经生长导向因子Slit2在鸡胚各发育时期神经管和脊髓的阳性表达呈动态变化。Slit2蛋白在脊髓发育过程起着重要作用。

Objective To explore the expression of neuronal guidance factor Slit2 in the neural tube and spinal cord of chick embryonic development. Method The protein expression pattern of Slit2 was detected in the neural tube（HH6-HH10） and spinal cord（3d-17d） using immunohistochemistry. Results Slit 2 positive product was detected in different stages of neural tube and spinal cord of chick embryo, superior in the spinal cord midline of neural floor plate to olher regions, especially on 9 day chick embryo, but showed an atlenualed and diffused trend after 11 days. Conclusion The expression pattern of neuronal guidance factor Slit2 in the neural tube and spinal cord of chick embryo presented a dynamic change.