摘要
目的建立胰岛素抵抗心肌细胞模型。方法原代培养2~3d SD大鼠心肌细胞分为对照组、葡萄糖组(glucose,G)、胰岛素组(insulin,Ins)、葡萄糖+胰岛素组(C+Ins)。在干预24h和48h后评价细胞的胰岛素敏感性,检测脂联素表达。结果干预48h后,Ins组、G+Ins组心肌细胞~3H-D-葡萄糖掺入率分别为(12.46±1.11)nmol·mg^(-1)·h^(-1)、(10.61±1.14)nmol·mg^(-1)·h^(-1),均较对照组的(14.06±0.43)nmol·mg^(-1)·h^(-1)显著下降,差异有统计学意义(P<0.05)。干预48h后,Ins组、G+Ins组心肌细胞脂联素mRNA表达分别为0.35±0.04、0.38±0.04,均较对照组的0.77±0.08显著下降,差异有统计学意义(P<0.05)。对照组、G组、Ins组、G+Ins组~3H-D-葡萄糖掺入率与心肌细胞脂联素mRNA表达量均呈显著正相关(r=0.92、0.82、0.89、0.86,P均<0.05)。结论应用高胰岛素诱导法、高葡萄糖+高胰岛素共同诱导法均可建立胰岛素抵抗心肌细胞模型;胰岛素抵抗下调心肌细胞脂联素表达水平;胰岛素抵抗心肌细胞脂联素表达水平与胰岛素抵抗呈正相关。
Objective To establish insulin resistance cardiocytes model. Methods Cardiocytes were cultured for 24h and 48h in vitro and were divided into four groups : control group( control), glucose group ( G), insulin group ( Ins ), glucose and insulin group ( G + Ins). The adiponectin ( APN ) expression in those groups was tested and the insulin sensitivity was evaluated by 3H -D -glucose mixing experiment. Results 3 H - D - glucose mixing rate in Ins group ( 12.46 ± 1.11 ) nmol · mg - 1 · h - l and G + Ins group (10.61 ± 1.14)nmol · mg-1· h-1 were all lower than control group( 14.06± 0.43 )nmol · mg-1· h-1 (P 〈 0.05 ). APN expression in Ins group 0.35 ±0.04, G + Ins group 0.38 ± 0.04, were all lower than control group 0.77 ± 0.08 after 48h( P 〈 0.05 ). 3H - D - glucose mixing rate in four groups was positively related to APN expression ( P 〈 0.05 ). Conclusion The insulin resistance cardiocytes model can be estabhshed by high insulin or high glucose + insulin. Insulin resistance down - regulates cardiocytes adiponectin expression. Insulin resistance is positively related to APN expression.
出处
《河北医科大学学报》
CAS
2012年第9期993-996,共4页
Journal of Hebei Medical University
基金
河北省卫生厅重大科技攻关项目(20100075)
关键词
肌细胞
心脏
胰岛素
模型
动物
myocytes, cardiac
insulin
models, animal