乙型肝炎病毒DNA与血清标志物的检测结果分析

THE ANALYSIS OF TEST RESULT BETWEEN VIRUS SERUM MARKERS AND NUCLEIC ACID OF HEPATITIS B VIRUS

目的探讨血清乙型肝炎病毒(hepatitis B virus,HBV)脱氧核糖核酸(deoxyribonucleic acid,DNA)水平与HBV血清标志物(HBV-marks,HBV-M)的相关性。方法采用实时荧光定量PCR对649例HBV感染者血清标本中HBV-DNA含量进行检测,同时用酶联免疫吸附测定(enzyme linked immuno sorbent assay,ELISA)法检测其HBV-M。结果 246例乙型肝炎e抗原(hepatitis B e antigen,HBeAg)阳性标本中HBV-DNA阳性率为100.0%,与HBeAg阴性标本的阳性率比较,差异有统计学意义(P<0.01);乙型肝炎表面抗原(hepatitis B santigen,HBsAg)阳性标本和HBsAg阴性标本,HBV-DNA检测结果比较差异有统计学意义(P<0.01)。结论患者血清HBV-DNA的阳性率与HBV-M的存在状态相关,采用实时荧光定量聚合酶链反应(fluorescent quantitativepolymerase chain reaction,FQ-PCR)法检测HBV-DNA能更准确、直接地反映体内病毒复制情况。

Objective To investigate the relationship between the level of hepatitis B virus （HBV） DNA and serum markers. Methods Fluorescent quantitative polymerase chain reaction （ FQ - PCR） was used to detect the contents of serum HBV - DNA in 649 patients infected HBV and enzyme linked immuno sorbent assay （ELISA） was used to detect the immunological markers. Results The positive rate of HBV - DNA was 100.0% in the hepatitis B e antigen （HBeAg） positive group. There was significant difference between HBeAg positive group and negative group（ P 〈 0.01 ）. The positive rate of HBV -DNA was also significantly different between the hepatitis B s antigen （HBsAg）positive group and negative group（P 〈 0.01 ）. Conclusion The positive rate of serum HBV - DNA is related to the mode of serum HBV markers. The result of serum HBV - DNA detected by FQ - PCR can reflect the replication of hepatitis B virus more correctly and directly.