五种重庆绿茶抑制致突变作用的研究

A STUDY ON THE ANTIMUTAGENESIS EFECCT OF FIVE KINDS OF CHONGQING GREEN TEA

本文采用沙门氏菌回复突变试验研究了五种重庆绿茶的三种样品制备形式对四种直接和间接致突变剂体外致突变性的抑制作用。结果表明,绿茶丙酮提取物和水浸浓缩液对黄曲霉毒素B_1(AFB_1)和苯并(a)芘(B(a)P)两种间接致突变剂有显著抑制作用,抑制率达到89％以上。且剂量效应关系十分明显。同时接近饮用浓度的水浸液对AFB_1和B(a)P的致突变性抑制率亦达到70%以上。但五种绿茶样品无论丙酮提取物还是水浸液对2,7—二氨基芴和叠氮钠两种直接致突变剂的诱变性均无明显抑制作用,抑制率都在20％以下。

This paper reports the antimutagenesis effect of three different preparations of five kinds of Chongqing green tea on four direct and indirect mutagens with Ames test.The results show that acetone extracts and concentrated water extracts of all the above green teas markedly inhibit the mutagenicity of AFB1 and B(a)P.The inhibition rate is above 89%, and the dose-response relation is clear. The inhibition rate of drinking concentration of water extract of the teas for mutagenicity of AFB1 and B(a) P is above 70%. But neither the acetone extracts nor the water extracts of the above green tea inhibit the mutagenicity of the two direct mutagens-2, 7-diaminofluorene and sodium azide, the inhibition rates are all below 20%. The possible mechanisms of action are being discussed