血清食物特异性抗体IgG和IgE检测对湿疹诊断的临床意义

Clinical significance of detection of food specific antibody IgG and IgE in serum for diagnosing eczema

目的探讨检测血清中食物特异性抗体IgE(sIgE)和IgG(sIgG)在诊断湿疹中的应用价值。方法对125例湿疹患者(实验组),采用免疫印迹法(Western blot)和酶联免疫法(ELISA)分别检测血清中食物sIgE和sIgG,并与35例健康献血者(对照组)进行比较。结果实验组中食物sIgG阳性率(43.2%)明显高于sIgE(20.8%),差异有统计学意义(P<0.01),与对照组sIgG(5.7%)和sIgE(8.6%)比较,差异均有统计学意义(P<0.01)。主要食物过敏原是牛奶和鸡蛋,其次为虾、蟹和鱼。联合检测sIgG和sIgE的阳性率高于单独检测sIgG或sIgE。结论食物sIgG和sIgE可能参与湿疹的发病,联合检测食物sIgG和sIgE可以提高湿疹血清学诊断的阳性率。

Objective To explore the clinical value of diagnosing eczema by detecting food specific antibodies IgE and IgG in ser- um of patients. Methods The serum levels of IgE（slgE） and IgG（slgG） from 215 patients of eczema（test group） were determined by Western-blot and ELISA and the results were compared with the outcome from the serum samples from 35 healthy blood donor （control group）. Results The positive rates of slgG and slgE in test group and control group were 43.2 %, 20.8% and 5.7 %, 8. 6% respectively. The positive rate of slgG was significant higher than slgE in test group（P〈0.01）. The positive rates of both slgG and slgE in test group were significant higher compared with the results of control group（P〈0.01）. The mainly allergen in serum of eczema patients were milk and egg, the secondly allergen include shrimp, crab and fish. The positive rates obtained by combined detection of slgG and slgE exceed obviously than detection of slgG or slgE alone. Conclusion Food specific antibodies slgE and slgG may be involved in the pathogenic progress of eczema. Combined detection of slgG and slgE contributes to improving positive rates of serum diagnosis for eczema.