EsA对BXSB狼疮性肾炎小鼠作用观察

Effect of Esculentoside A on therapeutic and cytokines secretion of lupus nephritis in BXSB mice

目的观察商陆皂苷甲(EsA)对BXSB小鼠狼疮性肾炎(LN)的治疗作用及IL-6及TNF-α分泌的影响,探讨EsA对LN的可能作用机制。方法 12周龄雄性BXSB小鼠24只,随机分成模型对照组、EsA低剂量治疗组和EsA高剂量治疗组。腹腔注射每日1次,4周后达治疗终点。结果 EsA能显著降低BXSB小鼠尿蛋白/尿肌酐比值,改善BXSB肾脏病变。结论 EsA对BXSB狼疮性肾炎小鼠具有显著治疗作用,抑制IL-6及TNF-α分泌产生可能参与EsA抗炎机制。

Objective To investigate the effect of Esculentoside A（EsA） on therapeutic and cytokines secretion in BXSB mice and the possible mechanism of EsA in treating lupus nephritis（LN）. Methods 24 male BXSB mice at the age of 12 weeks were ran- domly divided into 3 groups., untreated model group, EsA（5 mg ·kg^-1 . d^-1 ）-treated model group and EsA（20mg · kg · d^-1 ） treated model group. All mice at the age of 16 weeks were killed to obtain samples. Results EsA administration markedly alleviated urine protein/urine creatinine,improved pathological kidney damage and reduced the serum IL-6 and TNF-α level. Conclusion EsA may play significant roles in the treatment of BXSB mice through modulation on inflammatory cytokines.