摘要
目的:测量百草枯中毒后大鼠血清中炎症因子的水平。方法:将96只大鼠随机分为阴性对照组、高剂量染毒组(120mg/kg)、中剂量染毒组(60mg/kg)和低剂量染毒组(30mg/kg),每组24只大鼠。实验分8、24、72h三个时间段进行观察。应用酶联免疫吸附法(ELISA)测量血清TGF-β1、TNF-a、IL-10浓度。将大鼠肺进行病理切片及Masson染色观察肺纤维化情况。结果:随染毒时间延长,各剂量组细胞因子水平逐渐增加,24、72h组与对照组比较均有显著性差异(P<0.05)。72h高剂量组各指标与同时间点低剂量组比较均有显著性差异(P<0.05)。72h高、中、低3个剂量组与8h时间点比较有显著性差异(P<0.05)。大鼠肺组织中出现炎性粒细胞浸润,广泛弥漫的间质纤维化表现。Masson染色,24h后肺组织中分泌纤细的胶原纤维,72h后可见到大量蓝染的胶原纤维。结论:大鼠百草枯中毒后TGF-β1、TNF-a、IL-10等炎症因子水平显著升高。
Objective： To measure serum levels of inflammatory factors in paraquat poisoned rats. Meth- ods： 96 rats were grouped in accordance with the randomly principle. They were divided into control group and high dose exposure group （120mg/kg）, middle dose exposure group （60mg/kg） and low-dose exposure group （30mg/ kg）, n= 24 rats. Experimental were divided into8,24,72 h points for observation. Used enzyme linked immunosor- bent assay （ELISA） measurement to measure serum TGF-β1 , TNF-a, IL-10 concentration. The rats lung were used biopsy and Masson staining to observe pulmonary fibrosis. Results： With the exposure time, each dose group the levels of eytokines gradually increased. 24,72 h compared with the control group, the differences were statistically significant （P〈0.05 orP〈0.01）. The every target in 72h high dose group with the same point compared with low- dose group was significantly （P〈0.05）. 72h high, medium and low-dose group compared with the 8h time group was statistically significant （P〈0. 05）. Rat lung tissue infiltration appears inflammatory granuloeytes, extensive diffuse interstitial fibrosis of the performance. Conclusion： In paraquat poisoned rats, theinflammatory cytokine of TGF-β1 , TNF-a, IL-10 levels were significantly increased.
出处
《陕西医学杂志》
CAS
2012年第9期1107-1109,共3页
Shaanxi Medical Journal
基金
陕西省科学技术研究发展计划项目(No 2012k16-01-03)
