气相色谱法同时快速测定青贮饲料中的挥发性脂肪酸及乳酸

The Volatile Fatty Acids and Lactic Acid in Silage Determined Quickly by the Gas Chromatography

目前,国内外测定乳酸和挥发性脂肪酸(VFA)的方法主要是气相色谱法,一般情况下是对乳酸和挥发性脂肪酸分别进行测定,青贮饲料中乳酸和挥发性脂肪酸的同时测定未见报道。采用简便的甲酯衍生化方法,利用气相色谱填充柱,探索出一种能同时快速测定青贮饲料中乳酸和挥发性脂肪酸的方法,以期为青贮饲料的研究提供可靠的分析技术。

At present gas chromatography is considered as one of the most reliable methods in measuring lactic acid and volatile fatty acid domestically and abroad. Lactic acid and volatile fatty acid were determined respectively in general method. Lactic acid and volatile fatty acid were determined simultaneously in corn ensilage have not been reported. Using a simple and convenient method for derivative process of Methyl in this paper, a packed column was used in gas chromatography, exploring a new way of a rapid method for simultaneous determination of lactic acid and volatile fatty acid in corn ensilage to provide a reliable analysis technology for the research on corn ensilage.